Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR 97239, United States.
Behav Brain Res. 2013 Jan 15;237:207-14. doi: 10.1016/j.bbr.2012.09.029. Epub 2012 Sep 24.
Apolipoprotein E, which plays an important role in lipid transport and metabolism and neuronal repair, might modulate the CNS risk following (56)Fe irradiation exposure during space missions. In this study, we investigated this risk by behavioral and cognitive testing male E2, E3, and E4 mice 3 months following cranial (56)Fe irradiation. In the open field, mice irradiated with 2 Gy showed higher activity levels than sham-irradiated mice or mice irradiated with 1 Gy. In addition, E2 mice showed higher activity and lower measures of anxiety than E3 and E4 mice in the open field and elevated zero maze. During hidden platform training, sham-irradiated mice showed most robust learning, 1 Gy irradiated mice reduced learning, and 2 Gy irradiated mice no improvement over the four sessions. In the water maze probe trials, sham-irradiated E2, E3, and E4 mice and E2 and E4 mice irradiated with 1 Gy showed spatial memory retention, but E3 mice irradiated with 1 Gy, and E2, E3, and E4 mice irradiated with 2 Gy did not. Thus, cranial (56)Fe irradiation increases activity levels in the open field and impairs spatial learning and memory in the water maze. E3 mice are more susceptible than E2 or E4 mice to impairments in spatial memory retention in the water maze, indicating that apoE isoform modulates the CNS risk following space missions.
载脂蛋白 E 在脂质转运和代谢以及神经元修复中发挥重要作用，可能调节太空任务期间（56）Fe 辐照暴露后中枢神经系统的风险。在这项研究中，我们通过行为和认知测试 3 个月后接受颅（56）Fe 照射的雄性 E2、E3 和 E4 小鼠来研究这种风险。在开阔场中，2 Gy 照射的小鼠比假照射或 1 Gy 照射的小鼠表现出更高的活动水平。此外，E2 小鼠在开阔场和高架十字迷宫中比 E3 和 E4 小鼠表现出更高的活动水平和更低的焦虑水平。在隐藏平台训练中，假照射的小鼠表现出最强的学习能力，1 Gy 照射的小鼠学习能力降低，2 Gy 照射的小鼠在四个训练阶段均无改善。在水迷宫探测试验中，假照射的 E2、E3 和 E4 小鼠以及 1 Gy 照射的 E2 和 E4 小鼠保留了空间记忆，但 1 Gy 照射的 E3 小鼠和 2 Gy 照射的 E2、E3 和 E4 小鼠则没有。因此，颅（56）Fe 照射增加了开阔场中的活动水平，并损害了水迷宫中的空间学习和记忆。E3 小鼠比 E2 或 E4 小鼠更容易受到水迷宫中空间记忆保留受损的影响，表明载脂蛋白 E 异构体调节太空任务后的中枢神经系统风险。
