School of Medicine, Univ Federal de Juiz de Fora, MG, Brazil.
Braz Oral Res. 2012 Sep-Oct;26(5):443-9. doi: 10.1590/s1806-83242012000500011.
The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women.
本研究旨在使用荧光原位杂交（FISH）技术检验怀孕与非怀孕妇女龈下 8 种牙周致病菌定性和定量差异的假设。本横断面研究纳入了 20 名处于妊娠中期的孕妇和 20 名非孕妇。记录探诊深度、探诊出血、临床附着丧失和牙石情况。采集龈下菌斑样本，并用 FISH 技术检测伴放线放线杆菌、福赛坦纳菌、直肠弯曲菌、牙龈卟啉单胞菌、中间普氏菌、核梭杆菌、中间普氏菌和变黑普氏菌的存在和数量。应用曼-惠特尼 U 检验比较两组数据。两组的平均年龄、种族、婚姻状况、教育程度和经济水平相似。两组孕妇的临床参数无显著差异。尽管孕妇中间普氏菌的平均数量较高，但龈下牙周致病菌数量在两组间无显著差异。最常与牙周病相关的不同细菌的定植模式在孕妇和非孕妇的龈下菌斑中无差异。
