Scalioni Flávia, Carrada Camila, Machado Fernanda, Devito Karina, Ribeiro Luiz Cláudio, Cesar Dionéia, Ribeiro Rosangela
Universidade Federal de Juiz de Fora, Faculdade de Odontologia, Departamento de Odontopediatria, Juiz de Fora, MG, Brasil.
Universidade Federal de Minas Gerais, Faculdade de Odontologia, Belo Horizonte, MG, Brasil.
J Appl Oral Sci. 2017 May-Jun;25(3):250-257. doi: 10.1590/1678-7757-2016-0241.
To assess and compare dental caries experience and salivary S. mutans, S. sobrinus, and streptococci counts between groups of Down syndrome and non-Down syndrome children and adolescents.
This study included a sample of 30 Down syndrome children and adolescents (G-DS) and 30 age- and sex-matched non-Down syndrome subjects (G-ND). Dental caries experience was estimated by the number of decayed, missing, and filled teeth in the primary dentition and the permanent dentition. Unstimulated whole saliva samples were collected from all participants. The fluorescence in situ hybridization technique was used to identify the presence and counts of the bacteria. The statistical analysis included chi-square, Student's t-test and Spearman's correlation.
The G-DS exhibited a significantly higher caries-free rate (p<0.001) and a lower S. mutans salivary density (p<0.001). No significant differences were found in the salivary densities of S. sobrinus or streptococci between the groups (p=0.09 and p=0.21, respectively). The salivary S. mutans or S. sobrinus densities were not associated with dental caries experience in neither group.
The reduced dental caries experience observed in this group of Down syndrome children and adolescents cannot be attributed to lower salivary S. mutans densities, as determined with the fluorescence in situ hybridization technique.
评估和比较唐氏综合征与非唐氏综合征儿童及青少年群体之间的龋齿患病情况以及唾液中变形链球菌、远缘链球菌和链球菌的数量。
本研究纳入了30名唐氏综合征儿童及青少年（唐氏综合征组）和30名年龄及性别匹配的非唐氏综合征受试者（非唐氏综合征组）作为样本。通过乳牙列和恒牙列中龋、失、补牙的数量来评估龋齿患病情况。收集所有参与者未刺激的全唾液样本。采用荧光原位杂交技术鉴定细菌的存在及数量。统计分析包括卡方检验、学生t检验和Spearman相关性分析。
唐氏综合征组的无龋率显著更高（p<0.001），唾液中变形链球菌密度更低（p<0.001）。两组之间远缘链球菌或链球菌的唾液密度无显著差异（分别为p=0.09和p=0.21）。两组中唾液变形链球菌或远缘链球菌密度均与龋齿患病情况无关。
通过荧光原位杂交技术测定，在这组唐氏综合征儿童及青少年中观察到的龋齿患病情况减少不能归因于唾液中变形链球菌密度较低。
