Department of Biomolecular Engineering, University of California Santa Cruz, CA, USA.
RNA Biol. 2012 Sep;9(9):1155-60. doi: 10.4161/rna.21502. Epub 2012 Sep 1.
The RNA component of the RNase P complex is found throughout most branches of the tree of life and is principally responsible for removing the 5' leader sequence from pre-tRNA transcripts during tRNA maturation. RNase P RNA has a number of universal core features, however variations in sequence and structure found in homologs across the tree of life require multiple Rfam covariance search models to detect accurately. We describe a new Rfam search model to enable efficient detection of the diminutive archaeal Type T RNase P RNAs, which are missed by existing Rfam models. Using the new model, we establish effective score detection thresholds, and detect four new RNase P RNA genes in recently completed genomes from the crenarchaeal family Thermoproteaceae.
RNase P 复合物的 RNA 组分存在于生命之树的大多数分支中，主要负责在 tRNA 成熟过程中从前 tRNA 转录本中去除 5' 前导序列。RNase P RNA 具有许多通用的核心特征，但是在生命之树中的同源物中发现的序列和结构的变化需要多个 Rfam 协方差搜索模型来准确检测。我们描述了一个新的 Rfam 搜索模型，以能够有效地检测到微小的古菌 Type T RNase P RNA，这些 RNA 被现有的 Rfam 模型所忽略。使用新模型，我们建立了有效的得分检测阈值，并在最近完成的泉古菌科 Thermoproteaceae 的基因组中检测到了四个新的 RNase P RNA 基因。
