低密度脂蛋白氧化过程中α-生育酚的消耗
Alpha-tocopherol consumption during low-density-lipoprotein oxidation.
作者信息
Jessup W, Rankin S M, De Whalley C V, Hoult J R, Scott J, Leake D S
机构信息
Department of Biology and Biochemistry, Middlesex, U.K.
出版信息
Biochem J. 1990 Jan 15;265(2):399-405. doi: 10.1042/bj2650399.
Abstract
- The kinetics of the depletion of alpha-tocopherol in human low-density lipoprotein (LDL) were measured during macrophage-mediated and cell-free oxidation. The formation of oxidatively modified, high-uptake species of LDL in these systems was not detectable until all of the endogenous alpha-tocopherol had been consumed. 2. Supplementation of the alpha-tocopherol content of LDL by loading in vivo extended the duration of the lag period during which no detectable oxidative modification occurred. 3. The addition of a flavonoid (morin) prevented both alpha-tocopherol consumption and oxidative modification of LDL. 4. The alpha-tocopherol contents of LDLs from a range of individual donors could not be used to predict their relative resistance to oxidation, indicating that other endogenous antioxidants may also be present, and quantitatively significant, in human LDL.
摘要
- 在巨噬细胞介导的氧化和无细胞氧化过程中，测定了人低密度脂蛋白（LDL）中α-生育酚的消耗动力学。在这些系统中，直到所有内源性α-生育酚被消耗殆尽，才检测到氧化修饰的、高摄取性LDL物种的形成。2. 通过体内加载补充LDL的α-生育酚含量，延长了无可检测氧化修饰发生的滞后期持续时间。3. 添加一种类黄酮（桑色素）可防止α-生育酚的消耗和LDL的氧化修饰。4. 来自一系列个体供体的LDL的α-生育酚含量不能用于预测其相对抗氧化性，这表明人LDL中可能还存在其他内源性抗氧化剂，且在数量上具有重要意义。
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