氟铝酸盐在单个大鼠肝细胞中模拟激动剂的作用。
Fluoroaluminate mimics agonist application in single rat hepatocytes.
作者信息
Woods N M, Dixon C J, Cuthbertson K S, Cobbold P H
机构信息
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
出版信息
Biochem J. 1990 Jan 15;265(2):613-5. doi: 10.1042/bj2650613.
Abstract
Single rat hepatocytes microinjected with the photoprotein aequorin were stimulated with glycogenolytic agonists or low concentrations of fluoroaluminate. Both protocols resulted in the generation of oscillations in cytosolic free Ca2+ levels from a resting value of approx. 200 nM and peaking at over 600 nM. However, oscillations induced by receptor-dependent agonists were more regular in both frequency and time course than those induced by direct activation of G-proteins. The role of G-proteins in the generation of repetitive free Ca2+ oscillations is discussed.
摘要
用发光蛋白水母发光蛋白微注射的单个大鼠肝细胞，用糖原分解激动剂或低浓度氟铝酸盐刺激。两种方案均导致细胞质游离Ca2+水平从约200 nM的静息值开始振荡，并在超过600 nM时达到峰值。然而，受体依赖性激动剂诱导的振荡在频率和时间进程上比直接激活G蛋白诱导的振荡更规则。讨论了G蛋白在重复性游离Ca2+振荡产生中的作用。
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