佛波酯诱导的单个大鼠肝细胞中游离钙离子瞬变的改变。
Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes.
作者信息
Woods N M, Cuthbertson K S, Cobbold P H
机构信息
Department of Zoology, University of Liverpool, U.K.
出版信息
Biochem J. 1987 Sep 15;246(3):619-23. doi: 10.1042/bj2460619.
Abstract
The effect of the phorbol esters phorbol 12-myristate 13-acetate (TPA) and phorbol 12,13-dibutyrate (PDB) on changes in free Ca2+ concentration ([Ca2+]i) in single rat hepatocytes, microinjected with the photoprotein aequorin, were investigated. [Arg8]vasopressin and phenylephrine induced a series of repetitive [Ca2+]i transients. Phorbol esters inhibited the alpha 1-adrenoceptor-induced response; sub-nanomolar concentrations decreased the transient frequency, and higher concentrations abolished the transients. The inhibitory effect of PDB was readily reversible. Phorbol esters were less effective in decreasing the frequency of [Arg8]-vasopressin-induced transients, and the inhibition could be overcome by high [Arg8]vasopressin concentrations.
摘要
研究了佛波酯佛波醇12 -肉豆蔻酸酯13 -乙酸酯（TPA）和佛波醇12,13 -二丁酸酯（PDB）对显微注射了光蛋白水母发光蛋白的单个大鼠肝细胞内游离钙离子浓度（[Ca2+]i）变化的影响。[精氨酸8]加压素和去氧肾上腺素诱导了一系列重复性的[Ca2+]i瞬变。佛波酯抑制α1 -肾上腺素能受体诱导的反应；亚纳摩尔浓度降低瞬变频率，更高浓度则消除瞬变。PDB的抑制作用易于逆转。佛波酯在降低[精氨酸8] -加压素诱导的瞬变频率方面效果较差，且高浓度的[精氨酸8]加压素可克服这种抑制作用。
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J Biol Chem. 1982 Jul 10;257(13):7847-51.
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Inhibition of insulin receptor binding by phorbol esters.佛波酯对胰岛素受体结合的抑制作用。
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Inositol (1,4,5)trisphosphate-promoted Ca2+ release from microsomal fractions of rat liver.肌醇（1,4,5）三磷酸促进大鼠肝脏微粒体组分释放钙离子。
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myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.肌醇1,4,5-三磷酸。肝脏中激素动员细胞内钙离子的第二信使。
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