实时 RT-PCR 检测不同库蠓属种中的西马伦伯格病毒。
Detection of Schmallenberg virus in different Culicoides spp. by real-time RT-PCR.
机构信息
Veterinary and Agrochemical Research Centre, Brussel, Belgium.
出版信息
Transbound Emerg Dis. 2012 Dec;59(6):471-5. doi: 10.1111/tbed.12000. Epub 2012 Oct 1.
PMID:23025501
Abstract
To identify possible vectors of Schmallenberg virus (SBV), we tested pools containing heads of biting midges (Culicoides) that were caught during the summer and early autumn of 2011 at several places in Belgium by real-time RT-PCR. Pools of heads originating from following species: C. obsoletus complex, C. dewulfi and C. chiopterus were found positive, strongly indicating that these species are relevant vectors for SBV.
摘要
为了鉴定沙氏山病毒（SBV）可能的传播媒介，我们采用实时 RT-PCR 方法，检测了 2011 年夏末至初秋在比利时几个地点采集的叮咬蠓（库蠓属）头部样本。结果发现源自 C. obsoletus 复合种、C. dewulfi 和 C. chiopterus 的蠓头部样本呈阳性，这强烈表明这些物种是 SBV 的相关传播媒介。
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