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What parameters to consider and which software tools to use for target selection and molecular design of small interfering RNAs.

作者信息

Matveeva Olga

机构信息

Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.

出版信息

Methods Mol Biol. 2013;942:1-16. doi: 10.1007/978-1-62703-119-6_1.

DOI:10.1007/978-1-62703-119-6_1
PMID:23027043
Abstract

The design of small gene silencing RNAs with a high probability of being efficient still has some elements of an art, especially when the lowest concentration of small molecules needs to be utilized. The design of highly target-specific small interfering RNAs or short hairpin RNAs is even a greater challenging task. Some logical schemes and software tools that can be used for simplifying both tasks are presented here. In addition, sequence motifs and sequence composition biases of small interfering RNAs that have to be avoided because of specificity concerns are also detailed.

摘要

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