野油菜黄单胞菌外膜脂蛋白GumB的表达、纯化及结晶
Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris.
作者信息
Jacobs Melisa, Salinas Silvina R, Bianco María I, Ielpi Luis
机构信息
Laboratory of Bacterial Genetics, Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Oct 1;68(Pt 10):1255-8. doi: 10.1107/S1744309112036597. Epub 2012 Sep 29.
Abstract
GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris, is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction-quality crystals of native GumB were obtained. A complete data set was collected to 2.54 Å resolution with an R(p.i.m.) of 0.034. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 90.5, c = 120.7 Å.
摘要
GumB是一种预测的外膜脂蛋白，参与黄原胶的合成和/或分泌。这种由野油菜黄单胞菌产生的胞外多糖因其重要的流变学性质而在工业上具有重要价值。GumB结构的解析将为黄原胶的聚合和/或分泌机制提供深入了解。GumB经过过量表达和纯化，获得了天然GumB的衍射质量晶体。收集了完整的数据集，分辨率达到2.54 Å，R(p.i.m.)为0.034。晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 84.4，b = 90.5，c = 120.7 Å。
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