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Characterization of an endo-processive-type xyloglucanase having a β-1,4-glucan-binding module and an endo-type xyloglucanase from Streptomyces avermitilis.阿维链霉菌内切型木葡聚糖酶和具有β-1,4-葡聚糖结合模块的内切型木葡聚糖酶的特性研究。
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A revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases.基于底物特异性内切葡聚糖酶诱导的生物力学变化的初生细胞壁的修订结构。
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Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1,4-glucanase (GH12) from Aspergillus niveus.来自构巢曲霉的一种重组木葡聚糖特异性内切-β-1,4-葡聚糖酶（GH12）的功能表征与寡聚化
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来自野油菜黄单胞菌野油菜致病变种的一种新型木葡聚糖酶的结晶及初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of a new xyloglucanase from Xanthomonas campestris pv. campestris.

作者信息

de Araújo Evandro Ares, Tomazini Atílio, Kadowaki Marco Antonio Seiki, Murakami Mário Tyago, Polikarpov Igor

机构信息

Instituto de Física de São Carlos, Universidade de São Paulo, 400 Trabalhador Saocarlense, 13566-590 São Carlos, SP, Brazil.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):676-8. doi: 10.1107/S174430911301275X. Epub 2013 May 25.

DOI:10.1107/S174430911301275X
PMID:23722852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3668593/
Abstract

Xyloglucanases (Xghs) are important enzymes involved in xyloglucan modification and degradation. Xanthomonas campestris pv. campestris (Xcc) is a phytopathogenic bacterium which produces a large number of glycosyl hydrolases (GH), but has only one family 74 GH (Xcc-Xgh). This enzyme was overexpressed in Escherichia coli, purified and crystallized. Diffraction data sets were collected for the native enzyme and its complex with glucose to maximum resolutions of 2.0 and 2.1 Å, respectively. The data were indexed in a hexagonal crystal system with unit-cell parameters a = b = 153.4, c = 84.9 Å. As indicated by molecular-replacement solution, the crystals belonged to space group P6(1).

摘要

木葡聚糖酶（Xghs）是参与木葡聚糖修饰和降解的重要酶。野油菜黄单胞菌野油菜致病变种（Xcc）是一种植物致病细菌，它能产生大量糖基水解酶（GH），但只有一种74家族的GH（Xcc - Xgh）。该酶在大肠杆菌中过表达、纯化并结晶。分别收集了天然酶及其与葡萄糖复合物的衍射数据集，最高分辨率分别为2.0和2.1 Å。数据在六方晶体系统中索引，晶胞参数a = b = 153.4，c = 84.9 Å。如分子置换解析所示，晶体属于空间群P6(1)。