Cell Biology and Biophysics Unit , European Molecular Biology Laboratory-EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Biol Chem. 2012 Apr;393(4):235-48. doi: 10.1515/hsz-2011-0237.
Laser-mediated nanosurgery has become popular in the last decade because of the previously unexplored possibility of ablating biological material inside living cells with sub-micrometer precision. A number of publications have shown the potential applications of this technique, ranging from the dissection of sub-cellular structures to surgical ablations of whole cells or tissues in model systems such as Drosophila melanogaster or Danio rerio . In parallel, the recent development of micropatterning techniques has given cell biologists the possibility to shape cells and reproducibly organize the intracellular space. The integration of these two techniques has only recently started yet their combination has proven to be very interesting. The aim of this review is to present recent applications of laser nanosurgery in cell biology and to discuss the possible developments of this approach, particularly in combination with micropattern-mediated endomembrane organization.
激光介导的纳米手术在过去十年中变得流行，因为以前未知的用亚微米精度在活细胞内消融生物材料的可能性。许多出版物已经显示了这项技术的潜在应用，从亚细胞结构的剖析到在模型系统（如黑腹果蝇或斑马鱼）中对整个细胞或组织的手术消融。与此同时，最近微图案化技术的发展使细胞生物学家有可能塑造细胞并可重复地组织细胞内空间。这两种技术的整合最近才开始，但它们的结合已被证明非常有趣。本综述的目的是介绍激光纳米手术在细胞生物学中的最新应用，并讨论这种方法的可能发展，特别是与微图案介导的内膜组织相结合。
