Farkhondeh Tahere, Boskabady Mohammad Hossein, Koohi Mohammd Kazem, Sadeghi-Hashjin Goudarz, Moin Mostafa
Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Cardiovasc Hematol Disord Drug Targets. 2013 Mar 1;13(1):45-9. doi: 10.2174/1871529x11313010005.
This study was aimed to examine the effect of lead acetate on inflammatory biomarkers in blood of guinea pigs in comparison with sensitized animals. Thirty guinea pigs were randomly allocated into control (group C), sensitized (group S), and three Pb-exposed (groups 0.1M Pb, 0.2M Pb and 0.4M Pb). Animal sensitized after aerosolized-ovalbumin (OA) challenge. Pb-exposed groups inhaled 0.1M, 0.2M and 0.4M lead acetate for 1 h, three times a week for two weeks. Total and differential WBC counts, PLA2 activity and total protein levels were evaluated in blood of all animal groups. Serum PLA2 activity, total protein and total WBC number in sensitized and animal exposed to lead were significantly higher as compared to control group (p <0.05). When compared to control group, the percentages of eosinophil, nuetrophil and basophiles were also increased in sensitized guinea pigs (p <0.01). The percentage of eosinophil and basophile in animals exposed to high level of lead and that of basophile in animals exposed to middle lead concentration versus to control group significantly increased (p <0.05). In addition, the significant difference between the lymphocyte percentages of Pb-exposed groups and control group were decreased in concentration dependent manner. The findings of the present study indicated that exposure to inhaled lead acetate may lead to asthma-like disease.
本研究旨在比较醋酸铅对豚鼠血液中炎症生物标志物的影响与致敏动物的情况。30只豚鼠被随机分为对照组（C组）、致敏组（S组）和三个醋酸铅暴露组（0.1M醋酸铅组、0.2M醋酸铅组和0.4M醋酸铅组）。动物经雾化卵清蛋白（OA）激发后致敏。醋酸铅暴露组吸入0.1M、0.2M和0.4M醋酸铅1小时，每周三次，共两周。对所有动物组的血液进行白细胞总数和分类计数、磷脂酶A2（PLA2）活性及总蛋白水平评估。与对照组相比，致敏组和铅暴露动物的血清PLA2活性、总蛋白和白细胞总数显著升高（p<0.05）。与对照组相比，致敏豚鼠的嗜酸性粒细胞、中性粒细胞和嗜碱性粒细胞百分比也增加（p<0.01）。高剂量铅暴露动物的嗜酸性粒细胞和嗜碱性粒细胞百分比以及中剂量铅暴露动物的嗜碱性粒细胞百分比与对照组相比显著增加（p<0.05）。此外，醋酸铅暴露组与对照组淋巴细胞百分比的显著差异呈浓度依赖性降低。本研究结果表明，吸入醋酸铅可能导致类似哮喘的疾病。
