Farkhondeh Tahereh, Boskabady Mohammad Hossein, Kohi Mohammad Kazem, Sadeghi-Hashjin Goudarz, Moin Mostafa
Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran , Tehran , Iran .
Drug Chem Toxicol. 2014 Jul;37(3):329-35. doi: 10.3109/01480545.2013.866133. Epub 2013 Dec 12.
Serum inflammatory mediators and white blood cells (WBC) counts in the blood of sensitized and lead exposed guinea pigs were evaluated.
Guinea pigs were randomly allocated into control (C), sensitized (S) and sensitized groups exposed to three lead concentrations (0.1, 0.2 and 0.4 M) during (DS) and after sensitization (PS), (n = 6 for each group). Animals were sensitized by intra-peritoneal injection and aerosol inhalation of ovalbumin (OA). Serum total protein, PLA2, IgE, histamine, total and differential WBC counts of blood were evaluated.
Serum PLA2, total protein, IgE, histamine, total and differential WBC counts in all three sensitized groups were significantly increased, but the percentage of lymphocyte was decreased compared to control group (p < 0.05 to p < 0.001). Serum total protein and total WBC number in all animals exposed to lead as well as the eosinophil and histamine in animals exposed to highest lead concentration and IgE in sensitized groups exposed to lead after sensitization were significantly higher, but the lymphocyte in animals exposed to two higher lead concentrations was lower than non exposed sensitized group (p < 0.05 to p < 0.001). The changes in all parameters in lead exposed animals after sensitization were higher than those during sensitization which was statistically significant for total WBC count in the animals exposed to low lead concentration (p < 0.05).
Inhaled lead can increase serum total protein, PLA2, IgE and histamine levels, total and most differential WBC counts in sensitized animal which was more pronounced in animals exposed to lead after compared to those during sensitization.
对致敏和铅暴露豚鼠血液中的血清炎症介质和白细胞（WBC）计数进行评估。
将豚鼠随机分为对照组（C）、致敏组（S）以及在致敏期间（DS）和致敏后（PS）暴露于三种铅浓度（0.1、0.2和0.4 M）的致敏组，每组n = 6。通过腹腔注射和雾化吸入卵清蛋白（OA）使动物致敏。评估血清总蛋白、PLA2、IgE、组胺、血液中的白细胞总数和分类计数。
与对照组相比，所有三个致敏组的血清PLA2、总蛋白、IgE、组胺、白细胞总数和分类计数均显著增加，但淋巴细胞百分比降低（p < 0.05至p < 0.001）。所有铅暴露动物的血清总蛋白和白细胞总数，以及最高铅浓度暴露动物中的嗜酸性粒细胞和组胺，以及致敏后铅暴露致敏组中的IgE均显著升高，但暴露于两种较高铅浓度的动物中的淋巴细胞低于未暴露的致敏组（p < 0.05至p < 0.001）。致敏后铅暴露动物中所有参数的变化均高于致敏期间，这在低铅浓度暴露动物的白细胞总数方面具有统计学意义（p < 0.05）。
吸入铅可增加致敏动物的血清总蛋白、PLA2、IgE和组胺水平、白细胞总数和大多数分类计数，与致敏期间相比，致敏后铅暴露动物中更为明显。
