Kahn M, Barney N P, Briggs R M, Bloch K J, Allansmith M R
Eye Research Institute, Boston, MA 02114.
Invest Ophthalmol Vis Sci. 1990 Feb;31(2):258-61.
Dinitrophenyl (DNP) derivatives of various molecular weights were tested for their ability to elicit ocular anaphylaxis after topical application to the eye of immunized animals. Adult male Sprague-Dawley rats were immunized by intraperitoneal injection of DNP-Ascaris suum extracts and alum and were then skin-tested with DNP-bovine serum albumin on day 13 post-immunization to assess their sensitivity to the DNP hapten. On day 14, animals were challenged topically with DNP derivatives in one eye; PBS was applied to the contralateral, control eye. Animals were evaluated clinically, and ocular tissues were processed for histologic evaluation. The compounds used for topical ocular challenge included the DNP derivative of egg albumin (MW 43,500 D), soybean trypsin inhibitor (MW 20,080 D), insulin (MW 5733 D), B-chain insulin (MW 3496 D), and lysine (MW 478 D). Only di-DNP-lysine elicited clinical signs of redness, edema, and tearing and histologic evidence of mast cell degranulation. None of the other compounds, tested in solutions of either equal numbers of milligram per milliliter or equimolar concentrations, elicited ocular anaphylaxis after topical application. A compound of low molecular weight, less than 3496, is needed to elicit ocular anaphylaxis when applied topically.
对各种分子量的二硝基苯基（DNP）衍生物进行了测试，以评估其在局部应用于免疫动物眼部后引发眼部过敏反应的能力。成年雄性Sprague-Dawley大鼠通过腹腔注射DNP-猪蛔虫提取物和明矾进行免疫，然后在免疫后第13天用DNP-牛血清白蛋白进行皮肤测试，以评估它们对DNP半抗原的敏感性。在第14天，用DNP衍生物对动物的一只眼睛进行局部激发；对侧对照眼应用磷酸盐缓冲液（PBS）。对动物进行临床评估，并对眼部组织进行组织学评估。用于局部眼部激发的化合物包括卵清蛋白的DNP衍生物（分子量43,500 D）、大豆胰蛋白酶抑制剂（分子量20,080 D）、胰岛素（分子量5733 D）、胰岛素B链（分子量3496 D）和赖氨酸（分子量478 D）。只有二-DNP-赖氨酸引发了发红、水肿和流泪的临床症状以及肥大细胞脱颗粒的组织学证据。在每毫升毫克数相等或等摩尔浓度的溶液中测试的其他化合物，局部应用后均未引发眼部过敏反应。当局部应用时，需要一种分子量小于3496的低分子量化合物来引发眼部过敏反应。
