Trocmé S D, Bonini S, Barney N P, Bloch K J, Allansmith M R
Eye Research Institute of Retina Foundation, Boston, MA.
Curr Eye Res. 1988 May;7(5):437-43. doi: 10.3109/02713688809031795.
A cellular late-phase reaction is described in a rat model of topically induced ocular anaphylaxis. Rats were immunized with dinitrophenylated Ascaris suum extract and alum and were tested for active cutaneous anaphylaxis on day 13. Rats with a strong skin test response were selected for ocular challenge with di-DNP-lysine. Macroscopic observation and histologic evaluation were performed at 1, 6, and 24 h. In rats showing a moderate macroscopic ocular response at 1 h, mast cell degranulation was significantly increased at 1 h; no significant increase in eosinophils, neutrophils or lymphocytes was found in the conjunctive of these animals. In rats showing a marked macroscopic ocular response at 1 h, mast cell degranulation was significantly increased at 1 and 6 h; the number of eosinophils was significantly increased at 1 and 6 h, and of neutrophils at 6 h only. At 24 h, neutrophil and eosinophil numbers returned to baseline levels. There was no macroscopic evidence of a late-phase response in either group of animals. Our results suggest that, in keeping with earlier observations in human skin, a strong early response to antigen is required for the development of a late-phase ocular response in the rat.
在局部诱导的眼部过敏反应大鼠模型中描述了一种细胞晚期反应。用二硝基苯基化猪蛔虫提取物和明矾对大鼠进行免疫，并在第13天测试其主动皮肤过敏反应。选择皮肤试验反应强烈的大鼠用二 - DNP - 赖氨酸进行眼部激发。在1、6和24小时进行宏观观察和组织学评估。在1小时时表现出中度宏观眼部反应的大鼠中，肥大细胞脱颗粒在1小时时显著增加；在这些动物的结膜中未发现嗜酸性粒细胞、中性粒细胞或淋巴细胞有显著增加。在1小时时表现出明显宏观眼部反应的大鼠中，肥大细胞脱颗粒在1和6小时时显著增加；嗜酸性粒细胞数量在1和6小时时显著增加，中性粒细胞数量仅在6小时时增加。在24小时时，中性粒细胞和嗜酸性粒细胞数量恢复到基线水平。两组动物均无晚期反应的宏观证据。我们的结果表明，与早期在人体皮肤中的观察结果一致，大鼠眼部晚期反应的发生需要对抗原有强烈的早期反应。
