Barney N P, Kleinman R E, Trocmé S D, Bloch K J, Allansmith M R
Eye Research Institute of Retina Foundation, Boston, MA.
Curr Eye Res. 1988 Sep;7(9):843-8. doi: 10.3109/02713688808997241.
Attenuation of the rat conjunctival response by repeated topical challenge with dinitrophenyl (DNP) hapten was demonstrated in our study. Adult rats were immunized by intraperitoneal injections of dinitrophenylated Ascaris suum extract (DNP-Asc) and alum. Serum levels of anti-DNP homocytotropic antibody were determined by passive cutaneous anaphylaxis in rats prepared with antibody 48 hours earlier. In other animals, topical challenge was performed by applying N,N'-di-2,4-DNP-L-lysine (di-DNP-lysine) in phosphate-buffered saline (PBS) to one eye; PBS alone was applied to the fellow eye. The degree of conjunctival reaction was assessed clinically, and ocular tissues were processed for histological evaluation. The intensity of the conjunctival reaction and extent of mast cell degranulation were significantly greater after one challenge with di-DNP-lysine than after multiple challenges. In the multiple-challenge group, the contralateral eye remained responsive to a single challenge with di-DNP-lysine. These results may have implications for therapeutic interventions in ocular anaphylaxis.
在我们的研究中，已证实通过用二硝基苯基（DNP）半抗原反复进行局部激发可减轻大鼠结膜反应。成年大鼠通过腹腔注射二硝基苯基化猪蛔虫提取物（DNP-Asc）和明矾进行免疫。在48小时前制备好抗体的大鼠中，通过被动皮肤过敏反应测定血清抗DNP亲细胞性抗体水平。在其他动物中，通过将N，N'-二-2,4-DNP-L-赖氨酸（二-DNP-赖氨酸）溶于磷酸盐缓冲盐水（PBS）滴眼至一只眼进行局部激发；另一只眼仅滴注PBS。临床评估结膜反应程度，并对眼组织进行组织学评估。与多次激发后相比，用二-DNP-赖氨酸单次激发后结膜反应强度和肥大细胞脱颗粒程度明显更大。在多次激发组中，对侧眼对二-DNP-赖氨酸单次激发仍有反应。这些结果可能对眼部过敏反应的治疗干预有启示意义。
