Department Genome Modifications and Carcinogenesis (F020), Research Program Infection and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Int J Cancer. 2013 May 15;132(10):2395-403. doi: 10.1002/ijc.27891. Epub 2012 Oct 25.
Of the 120 known human papillomaviruses (HPV), 51 HPV types infect the genital mucosa. Very little is known about the prevalence and viral load of the majority of these low-risk (Lr-) HPV types in screening populations. We determined the prevalence of 51 HPV types and three subtypes in 999 consecutive BD-SurePath™ liquid-based cervical cytology samples collected during routine gynecological health checks from Belgian women. This series of screening samples was enriched with ASC-US (n = 100), low-grade squamous intraepithelial lesion LSIL (n = 100) and high-grade squamous intraepithelial lesion (HSIL) (n = 97) and analyzed by BSGP5+/6+-PCR/MPG assay for 51 HPV types and three subtypes. In consecutive screening samples, any of the 54 genital HPV (sub)types was found in 37.1%; Hr-HPV types were detected more frequently (26.8%) than the 31 Lr-HPV types (16.4%) and the six possibly high-risk types (6.6%). High viral load infections were present in 17.0% of the screening population. Among the women with cytological abnormalities, the prevalence of high viral loads of Hr-HPV types increased from negative for intraepithelial lesion or malignancy (NIL/M) over ASC-US, LSIL to HSIL (5.3, 47.1, 84.2 and 91.8%, respectively). The prevalence of possibly Hr and Lr-HPV types increased from NIL/M to LSIL but declined to HSIL. From NIL/M to HSIL, Hr-HPV infections showed an increasing frequency of high viral loads compared to total DNA positivity, but the increase between LSIL and HSIL was small. Type-specific analyses revealed substantial differences between individual HPV types in these groups. Our study provides quantitative data for the whole spectrum of genital HPV in a Belgian screening population and in a representative set of women with cervical abnormalities.
在 120 种已知的人类乳头瘤病毒（HPV）中，有 51 种 HPV 类型感染生殖道黏膜。对于筛查人群中大多数低风险（Lr-）HPV 类型的流行率和病毒载量，我们知之甚少。我们确定了在 999 例连续的 BD-SurePath™液基宫颈细胞学样本中 51 种 HPV 类型和三种亚型的流行率，这些样本是在比利时女性常规妇科健康检查中收集的。这个筛查样本系列通过 BSGP5+/6+-PCR/MPG 检测 51 种 HPV 类型和三种亚型进行了补充，包括 ASC-US（n = 100）、低级别鳞状上皮内病变（LSIL）（n = 100）和高级别鳞状上皮内病变（HSIL）（n = 97）。在连续的筛查样本中，任何一种 54 种生殖道 HPV（亚）型的检出率为 37.1%；高危型 HPV 类型的检出率（26.8%）高于 31 种低危型 HPV 类型（16.4%）和 6 种可能高危型 HPV 类型（6.6%）。17.0%的筛查人群存在高病毒载量感染。在有细胞学异常的女性中，高危型 HPV 类型的高病毒载量患病率从无上皮内病变或恶性肿瘤（NIL/M）到 ASC-US、LSIL 再到 HSIL 逐渐增加（分别为 5.3%、47.1%、84.2%和 91.8%）。可能高危型和低危型 HPV 类型的患病率从 NIL/M 到 LSIL 增加，但到 HSIL 则下降。从 NIL/M 到 HSIL，与总 DNA 阳性相比，高危型 HPV 感染的高病毒载量频率增加，但 LSIL 到 HSIL 的增加幅度较小。针对特定 HPV 类型的分析显示，在这些组中，各 HPV 类型之间存在显著差异。我们的研究为比利时筛查人群和具有宫颈异常的代表性女性人群提供了生殖道 HPV 的全谱定量数据。
