Department of Pathology, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa.
Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa.
Viruses. 2023 Mar 15;15(3):758. doi: 10.3390/v15030758.
The interaction between cervicovaginal virome, bacteriome and genital inflammation has not been extensively investigated. We assessed the vaginal DNA virome from 33 South African adolescents (15-19 years old) using shotgun DNA sequencing of purified virions. We present analyses of eukaryote-infecting DNA viruses, with a focus on human papillomavirus (HPV) genomes and relate these to the vaginal bacterial microbiota (assessed by 16S rRNA gene sequencing) and cytokines (assessed by Luminex). The DNA virome included single-stranded (, ) and double-stranded DNA viruses (, , , , , , ). We identified 110 unique, complete HPV genomes within two genera ( and ) representing 40 HPV types and 12 species. Of the 40 HPV types identified, 35 showed positive co-infection patterns with at least one other type, mainly HPV-16. HPV-35, a high-risk genotype currently not targeted by available vaccines, was the most prevalent HPV type identified in this cohort. Bacterial taxa commonly associated with bacterial vaginosis also correlated with the presence of HPV. Bacterial vaginosis, rather than HPV, was associated with increased genital inflammation. This study lays the foundation for future work characterizing the vaginal virome and its role in women's health.
宫颈阴道病毒组、细菌组和生殖器炎症之间的相互作用尚未得到广泛研究。我们使用纯化病毒粒子的鸟枪法 DNA 测序，评估了来自 33 名南非青少年（15-19 岁）的阴道 DNA 病毒组。我们分析了真核感染 DNA 病毒，重点是人类乳头瘤病毒（HPV）基因组，并将这些与阴道细菌微生物组（通过 16S rRNA 基因测序评估）和细胞因子（通过 Luminex 评估）相关联。DNA 病毒组包括单链（、）和双链 DNA 病毒（、、、、、、）。我们在两个属（和）内鉴定了 110 个独特的、完整的 HPV 基因组，代表 40 种 HPV 类型和 12 个种。在所鉴定的 40 种 HPV 类型中，35 种与至少一种其他类型呈阳性共感染模式，主要是 HPV-16。HPV-35 是一种目前不受现有疫苗靶向的高危基因型，是该队列中鉴定出的最常见 HPV 类型。与细菌性阴道病常见相关的细菌分类群也与 HPV 的存在相关。细菌性阴道病而不是 HPV 与生殖器炎症的增加相关。这项研究为进一步研究阴道病毒组及其在女性健康中的作用奠定了基础。
