Manosroi Aranya, Kumguan Kulthida, Chankhampan Charinya, Manosroi Worapaka, Manosroi Jiradej
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
J Nanosci Nanotechnol. 2012 Sep;12(9):7187-97. doi: 10.1166/jnn.2012.6500.
Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as the MMP-2 inhibition activity on human skin fibroblasts for anti-aging evaluation. The hot water crude extract showed the highest antioxidative activities (DPPH radical scavenging, metal ion chelating and lipid peroxidation inhibition) with the SC50, CC50 and IPC50 values of 5.40 +/- 1.23, 32.31 +/- 0.84 and 3.29 +/- 0.30 mg/ml, respectively, and the highest tyrosinase inhibition activity with the IC50 value of 0.49 +/- 0.23 mg/ml. The extract also showed no cytotoxicity on human skin fibroblasts with the cell viability of 80.52 +/- 15.16%. It demonstrated the anti-aging potential by having the pro and active MMP-2 inhibition activity, but lower than ascorbic acid of 1.28 and 1.12 times, respectively. The semi-purified extracts were prepared from this crude extract by solvent-solvent partition. The ethyl acetate soluble fraction showed higher activities (DPPH radical scavenging, metal ion chelating and tyrosinase inhibition) than the crude extract of 23.48, 71.80 and 2.58 times, respectively. This fraction exhibited similar pro and active MMP-2 inhibitory effect to the crude extract. The results from this study have indicated the possible application of the ethyl acetate fraction of the hot water crude extract from leaves of Longkong to be developed as an anti-aging product.
从泰国尖竹汶采集的龙功叶，采用热水和冷水工艺，使用水、氯仿和甲醇三种不同溶剂进行提取。对粗提物进行抗氧化活性、酪氨酸酶抑制、体外细胞毒性以及对人皮肤成纤维细胞的基质金属蛋白酶 -2（MMP-2）抑制活性测试，以进行抗老化评估。热水粗提物表现出最高的抗氧化活性（DPPH 自由基清除、金属离子螯合和脂质过氧化抑制），其 SC50、CC50 和 IPC50 值分别为 5.40±1.23、32.31±0.84 和 3.29±0.30 mg/ml，并且具有最高的酪氨酸酶抑制活性，IC50 值为 0.49±0.23 mg/ml。该提取物对人皮肤成纤维细胞也无细胞毒性，细胞活力为 80.52±15.16%。它通过具有前 MMP-2 和活性 MMP-2 抑制活性显示出抗老化潜力，但分别比抗坏血酸低 1.28 倍和 1.12 倍。通过溶剂 - 溶剂分配从该粗提物制备了半纯化提取物。乙酸乙酯可溶级分显示出比粗提物更高的活性（DPPH 自由基清除、金属离子螯合和酪氨酸酶抑制），分别是粗提物的 23.48、71.80 和 2.58 倍。该级分对前 MMP-2 和活性 MMP-2 的抑制作用与粗提物相似。本研究结果表明，龙功叶热水粗提物的乙酸乙酯级分有可能开发成为一种抗老化产品。
