Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Guangzhou 510380, China.
Gene. 2012 Dec 15;511(2):320-5. doi: 10.1016/j.gene.2012.09.087. Epub 2012 Oct 2.
The present study examined the genetic variation of the family Osteoglossidae from different geographical locations based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) and ATPase subunit 6 (ATPase6) genes; we then re-constructed the phylogenetic relationships using the two sequences in combination. The results showed that the partial sequences of mitochondrial ND2 and ATPase6 of the family Osteoglossidae were 813 bp and 669 bp, respectively. A total of 42 species-specific nucleotide positions of the family Osteoglossidae were found to be useful for molecular identification. The sequence variation showed greater differences (8.3%28.1% for the combined sequences, 8.3%26.7% for the ND2 gene, and 9.3%~28.7% for the ATPase6 gene) among the different species of Osteoglossidae, and there was a significant association between the genetic difference and geographical location. Phylogenetic analyses using neighbor-joining, Bayesian inference, and maximum parsimony (MP) methods based on the combined sequences of the two genes were able to distinguish the different species and were in agreement with the existing taxonomy based on morphological characters and in association with the geographical distribution among seven species of the family Osteoglossidae.
本研究基于线粒体 NADH 脱氢酶亚单位 2（ND2）和 ATP 合酶亚单位 6（ATPase6）基因，对来自不同地理区域的骨舌鱼科鱼类的遗传变异进行了研究；然后，我们使用这两个序列的组合重新构建了系统发育关系。结果表明，骨舌鱼科鱼类的线粒体 ND2 和 ATPase6 部分序列分别为 813bp 和 669bp。共发现 42 个种特异性核苷酸位置对骨舌鱼科鱼类的分子鉴定有用。序列变异显示出较大的差异（组合序列的差异为 8.3%28.1%，ND2 基因的差异为 8.3%26.7%，ATPase6 基因的差异为 9.3%~28.7%），不同的骨舌鱼科鱼类之间存在显著的遗传差异与地理位置的关联。基于两个基因的组合序列的邻接法、贝叶斯推断和最大简约法（MP）的系统发育分析能够区分不同的物种，并与基于形态特征的现有分类学以及与 7 种骨舌鱼科鱼类的地理分布相一致。
