Division of Neonatology, Department of Pediatrics, University of California San Diego Medical Center, San Diego, California 92103-8774, USA.
Breastfeed Med. 2013 Apr;8:205-9. doi: 10.1089/bfm.2012.0007. Epub 2012 Oct 5.
We hypothesized that pooling a mother's expressed breastmilk for 24 hours compared with individual pump session collection of milk would provide a more consistent caloric product without increasing bacterial contamination.
We investigated 24-hour pooled breastmilk collection by enrolling 19 mothers who were expressing milk for their infants. Mothers followed a standardized milk collection protocol for 4 study days: daily milk was pooled in a sterile 1-L bottle on Day 1, and on Day 2 milk was aliquoted for each pump session into a sterile 120-mL container. The next week the order of collection was reversed. Milk samples were plated, incubated, and evaluated for bacteria colonization. Milk samples were analyzed for protein, fat, and carbohydrate content.
There was inherently less variability in the caloric and nutrient content of pooled milk compared with individual samples, in which caloric density varied by as much as 29%. Mother's milk had highly variable bacterial counts ranging from 0 to greater than 100,000 colonies/mL. High bacteria counts (>100,000 colonies/mL) occurred in 14.7% (31 of 211) of individual samples compared with 8.6% (three of 35) of pooled samples (p=0.39).
Twenty-four-hour pooling of human milk reduces nutrient and caloric variability without increasing bacterial counts.
我们假设与单次泵吸收集的母乳相比，将母亲的母乳在 24 小时内进行混合收集，可提供更稳定的热量，同时不会增加细菌污染。
我们招募了 19 位正在用吸奶器吸奶喂养婴儿的母亲，对 24 小时混合母乳收集进行了研究。母亲遵循标准化的吸奶收集方案，进行 4 天的研究：第 1 天，将每日母乳在无菌 1 升瓶中混合；第 2 天，将母乳按照每次泵吸的量分装到无菌 120 毫升容器中。下周的收集顺序颠倒。对母乳样本进行平板培养，评估细菌定植情况。对母乳样本进行蛋白质、脂肪和碳水化合物含量分析。
与单独样本相比，混合母乳的热量和营养成分固有地更稳定，热量密度的变化最大可达 29%。母亲的母乳中细菌计数高度可变，范围从 0 到超过 100,000 个菌落/毫升。单个样本中细菌计数高（>100,000 个菌落/毫升）的情况占 14.7%（211 个中的 31 个），而混合样本中占 8.6%（35 个中的 3 个）（p=0.39）。
24 小时混合收集母乳可减少营养成分和热量的变异性，而不会增加细菌计数。
