Animal & Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
J Insect Physiol. 2012 Dec;58(12):1556-61. doi: 10.1016/j.jinsphys.2012.09.009. Epub 2012 Oct 4.
Understanding the evolutionary ecology of immune responses to persistent infection could provide fundamental insight into temporal dynamics or interactive mechanisms that could be co-opted for antibiotic treatment regimes. Additionally, identification of novel molecules involved in these processes could provide novel compounds for biotechnological development. The beetle Tenebrio molitor displays a high level of induced antimicrobial activity coincident with persistent immuno-resistant Staphylococcus aureus, and is the first invertebrate model for persistent infection. Here we present expressed sequence tags (ESTs) detected by suppression-subtraction hybridization of Tenebrio larvae after infection with S. aureus. Amongst others, we identified mRNAs coding for various oxidative enzymes and two antimicrobial peptides. These ESTs provide a foundation for mechanistic study of Tenebrio's immune system.
了解对持续感染的免疫反应的进化生态学可以为抗生素治疗方案提供有关时间动态或相互作用机制的基本见解。此外，鉴定这些过程中涉及的新分子可以为生物技术的发展提供新的化合物。甲虫黄粉虫在与持续免疫抗性金黄色葡萄球菌一致的情况下显示出高水平的诱导性抗菌活性，并且是第一个持续感染的无脊椎动物模型。在这里，我们介绍了通过金黄色葡萄球菌感染后的 Tenebrio 幼虫抑制差减杂交检测到的表达序列标签（EST）。除其他外，我们鉴定了编码各种氧化酶和两种抗菌肽的 mRNA。这些 EST 为 Tenebrio 免疫系统的机制研究提供了基础。
