Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Av. Sen Salgado Filho, No. 3000, Natal, RN, Brazil.
Carbohydr Polym. 2013 Jan 2;91(1):467-75. doi: 10.1016/j.carbpol.2012.07.075. Epub 2012 Aug 3.
Fucan (SV1) sulfated polysaccharides from the brown algae Sargassum vulgare were extracted, fractionated in acetone and examined with respect to chemical composition, anticoagulant, anti-inflammatory, antithrombotic effects and cellular proliferation. These polysaccharides contain low levels of protein, high level of carbohydrate and sulfate. Monosaccharides analysis revealed that SV1 was composed of fucose, galactose, xylose, glucuronic acid and mannose. SV1 polysaccharide prolonged activated partial thromboplastin time (aPTT) and exhibited high antithrombotic action in vivo, with a concentration ten times higher than heparin activity. PSV1, a purified form in gel filtration showed very low biological activities. SV1 stimulated the enzymatic activity of FXa. Its action on DPPH radical scavenging activity was 22%. This polymer has no cytotoxic action (hemolytic) on ABO and Rh blood types in different erythrocyte groups. It displays strong anti-inflammatory action at all concentrations tested in the carrageenan-induced paw edema model, demonstrated by reduced edema and cellular infiltration.
从褐藻马尾藻中提取的岩藻糖（SV1）硫酸化多糖，用丙酮进行分级，并对其化学组成、抗凝、抗炎、抗血栓形成作用和细胞增殖进行了研究。这些多糖的蛋白质含量低，碳水化合物和硫酸盐含量高。单糖分析表明，SV1 由岩藻糖、半乳糖、木糖、葡萄糖醛酸和甘露糖组成。SV1 多糖延长了活化部分凝血活酶时间（aPTT），并在体内表现出高抗血栓形成作用，其浓度比肝素活性高十倍。在凝胶过滤中得到的纯化形式 PSV1 显示出非常低的生物活性。SV1 刺激 FXa 的酶活性。其对 DPPH 自由基清除活性为 22%。该聚合物对 ABO 和 Rh 血型的不同红细胞组没有细胞毒性（溶血）作用。在角叉菜胶诱导的爪肿胀模型中，该聚合物在所有测试浓度下均表现出强烈的抗炎作用，通过减少水肿和细胞浸润来证明。
