Garaj-Vrhovac V, Horvat D, Koren Z
Institute for Medical Research and Occupational Health, University of Zagreb, Yugoslavia.
Mutat Res. 1990 Feb;243(2):87-93. doi: 10.1016/0165-7992(90)90028-i.
Cultured V79 Chinese hamster cells were exposed to continuous radiation, frequency 7.7 GHz, power density 30 mW/cm2 for 15, 30, and 60 min. The parameters investigated were the incorporation of [3H]thymidine and the frequency of chromosome aberrations. Data obtained by 2 methods (the incorporation of [3H]thymidine into DNA and autoradiography) showed that the inhibition of [3H]thymidine incorporation took place by complete prevention of DNA from entering into the S phase. The normal rate of incorporation of [3H]thymidine was recovered within 1 generation cycle of V79 cells. Mutagenic tests performed concurrently showed that even DNA macromolecules were involved in the process. In comparison with the control samples there was a higher frequency of specific chromosome lesions in cells that had been irradiated. Results discussed in this study suggest that microwave radiation causes changes in the synthesis as well as in the structure of DNA molecules.
将培养的V79中国仓鼠细胞暴露于频率为7.7 GHz、功率密度为30 mW/cm²的连续辐射下15、30和60分钟。所研究的参数是[³H]胸腺嘧啶核苷的掺入和染色体畸变频率。通过两种方法（[³H]胸腺嘧啶核苷掺入DNA和放射自显影）获得的数据表明，[³H]胸腺嘧啶核苷掺入的抑制是通过完全阻止DNA进入S期发生的。[³H]胸腺嘧啶核苷的正常掺入率在V79细胞的1个世代周期内恢复。同时进行的诱变试验表明，即使DNA大分子也参与了这一过程。与对照样品相比，受过辐照的细胞中特定染色体损伤的频率更高。本研究中讨论的结果表明，微波辐射会导致DNA分子的合成以及结构发生变化。
