General University Hospital and 1st Faculty of Medicine of Charles University in Prague, U nemocnice 2, 128 00, Prague 2, Czech Republic.
J Assist Reprod Genet. 2012 Nov;29(11):1207-12. doi: 10.1007/s10815-012-9853-4. Epub 2012 Sep 28.
To assess levels of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) in follicular fluids and sera of female patients undergoing in vitro fertilization (IVF) treatment, and discover the role of MMPs in IVF outcome prediction.
Sera and follicular fluids were obtained from 58 female patients treated for infertility by IVF. Twenty-nine of them became pregnant after the embryo-transfer; another 29 were not successful in IVF and did not conceive. Forty female non-pregnant blood donors and 38 healthy pregnant patients in the first trimester after the physiological conception were examined as control groups. MMP-2 and MMP-9 were quantitively assessed using enzyme-linked immunosorbent assay.
IVF females successfully conceiving after the IVF have shown the highest MMP-9 concentrations in sera (833.5 {686.0;958.7} ng/mL) and follicular fluids (9.6 {6.0; 17.0} ng/mL) compared to all other examined cohorts. Different behavior of MMP-2 and MMP-9 during the artificial ovulation was confirmed, because only MMP-9 has shown a vast difference between serum and follicular concentrations.
MMP-9 could be a good predictor of the successful IVF outcome (pregnancy), which was proven for serum as well as follicular MMP-9 levels.
评估接受体外受精 (IVF) 治疗的女性患者的卵泡液和血清中基质金属蛋白酶 2 和 9 (MMP-2 和 MMP-9) 的水平，并发现 MMP 在 IVF 结局预测中的作用。
收集 58 名因不孕接受 IVF 治疗的女性患者的血清和卵泡液。其中 29 例胚胎移植后妊娠；另外 29 例 IVF 未成功受孕。40 名非妊娠女性献血者和 38 名生理妊娠后第一 trimester 的健康孕妇作为对照组。采用酶联免疫吸附试验定量评估 MMP-2 和 MMP-9。
与所有其他检查队列相比，IVF 后成功受孕的 IVF 女性血清（833.5{686.0；958.7}ng/mL）和卵泡液（9.6{6.0；17.0}ng/mL）中 MMP-9 浓度最高。证实了 MMP-2 和 MMP-9 在人工排卵期间的不同行为，因为只有 MMP-9 显示了血清和卵泡浓度之间的巨大差异。
MMP-9 可能是 IVF 成功结局（妊娠）的良好预测因子，这在血清和卵泡 MMP-9 水平中均得到证实。
