Department of Food Science and Engineering, School of Science and Engineering, Jinan University, Number 601 Huangpu Road West, Guangzhou 510632, People's Republic of China.
J Agric Food Chem. 2012 Oct 31;60(43):10844-9. doi: 10.1021/jf303595e. Epub 2012 Oct 18.
This study enzymatically acrylates two flavonoids from bamboo-leaf extracts, isoorientin and isovitexin, with different fatty acids as acyl donors using Candida antarctica lipase B (CALB). The conversion yield ranged from 35 to 80% for fatty acids with different chain lengths. Higher isoorientin and isovitexin conversion yields (>75%) were obtained using lauric acid in tert-amyl-alcohol as the reaction medium. (1)H and (13)C nuclear magnetic resonance spectroscopy analysis showed that, in the presence of CALB, acylation occurred at the isoorientin and isovitexin primary hydroxyl group of glucose moiety and only monoesters were detected. Introducing an acyl group into isoorientin and isovitexin significantly improved their lipophilicity but reduced their antiradical activity.
本研究使用南极假丝酵母脂肪酶 B（CALB）将竹叶提取物中的两种黄酮类化合物异荭草苷和荭草苷分别与不同的脂肪酸进行酶促酯化，作为酰基供体。不同链长脂肪酸的转化率范围为 35%至 80%。在叔戊醇中使用月桂酸作为反应介质时，异荭草苷和荭草苷的转化率更高（>75%）。（1）H 和（13）C 核磁共振波谱分析表明，在 CALB 的存在下，酰化发生在异荭草苷和荭草苷葡萄糖部分的伯羟基上，仅检测到单酯。将酰基引入异荭草苷和荭草苷中显著提高了它们的亲脂性，但降低了它们的抗氧化活性。
