Kirkland J L, Hollenberg C H, Gillon W S
Institute of Medical Science, University of Toronto, Ontario, Canada.
Am J Physiol. 1990 Feb;258(2 Pt 1):C206-10. doi: 10.1152/ajpcell.1990.258.2.C206.
The effects of donor age and anatomic site on cellular replication and differentiation were studied in adipocyte precursors cloned from epididymal and perirenal depots of young, middle-aged, and senescent rats. As animals aged from 3 to 29 mo, there was a progressive reduction in the proportion of cells capable of extensive replication in both depots. An inverse relation between clonal capacity for replication and differentiation was found. This relation was affected by donor site but not age. Aging was, however, associated with a reduction in the frequency of clones capable of full differentiation into cells with single, large, central lipid inclusions. Hence, age and donor site may affect adipocyte precursor replication and differentiation by different mechanisms.
研究了供体年龄和解剖部位对从年轻、中年和老年大鼠附睾和肾周脂肪库克隆的脂肪细胞前体细胞复制和分化的影响。随着动物从3个月龄到29个月龄，两个脂肪库中能够广泛复制的细胞比例逐渐降低。发现克隆复制能力与分化之间呈负相关。这种关系受供体部位影响，但不受年龄影响。然而，衰老与能够完全分化为具有单个大的中央脂质包涵体的细胞的克隆频率降低有关。因此，年龄和供体部位可能通过不同机制影响脂肪细胞前体的复制和分化。
