Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran.
Department of Pathology, School of Medicine Birjand University of Medical Sciences, Birjand, Iran.
Curr Mol Med. 2024;24(5):676-682. doi: 10.2174/0115665240251646230919100920.
This study aimed to evaluate the effect of Chlorpyrifos (CPF) in rat heart tissue and the effect of Curcumin (Cur) on cardiac enzymes, oxidative indices, and histopathological changes in the cardiac tissue.
CPF, the most used organophosphorus pesticide (OP), has been reported to induce cardiotoxic effects.
The cardioprotective effects of Cur against CPF-induced toxicity have not been entirely investigated till now.
Forty male Wistar rats were randomized into five groups (n=8). C group (Control animals that received olive oil), CPF group (10 mg/kg/day), CPF + Cur 25, CPF + Cur 50, and CPF + Cur 100 groups (animals received 10 mg/kg/day CPF and 25, 50, and 100 mg/kg Cur, respectively). All treatments were administered oral gavage for 90 days. Cardiac enzymes (LDH & CPK) and oxidative stress (OS) biomarkers in heart tissue (malondialdehyde, Superoxide Dismutase) were measured. Histopathological changes in the heart tissue were also evaluated.
Chronic exposure to CPF significantly increased cardiac enzyme levels and OS biomarkers. Histological changes were found, including disorganization of the cardiac muscle fibers with disorganization and degeneration in myocardial fibers with separation of myofibrils and cytoplasmic vacuolization of cardiac muscle fibers. Administration of Cur (100 mg/kg) reversed serum LDH concentration and OS biomarkers to normal levels in CPF-exposed animals (p < 0.05) and significantly improved cardiac damage.
According to the results of this study, Cur can reduce the adverse effects of long-term exposure to CPF in rat heart tissue by modulating OS.
本研究旨在评估毒死蜱（CPF）对大鼠心脏组织的影响，以及姜黄素（Cur）对心肌酶、氧化指标和心脏组织组织学变化的影响。
CPF 是最常用的有机磷农药（OP）之一，据报道它会引起心脏毒性作用。
姜黄素对 CPF 诱导的毒性的心脏保护作用尚未得到全面研究。
40 只雄性 Wistar 大鼠随机分为五组（n=8）。C 组（对照组，给予橄榄油）、CPF 组（10mg/kg/天）、CPF+Cur25 组、CPF+Cur50 组和 CPF+Cur100 组（分别给予 10mg/kg/天 CPF 和 25、50 和 100mg/kg Cur）。所有治疗均通过口服灌胃给药，共 90 天。测量心脏组织中的心肌酶（LDH 和 CPK）和氧化应激（OS）生物标志物（丙二醛、超氧化物歧化酶）。还评估了心脏组织的组织学变化。
慢性暴露于 CPF 显著增加了心肌酶水平和 OS 生物标志物。发现组织学变化，包括心肌纤维排列紊乱，心肌纤维变性和分离，肌原纤维细胞质空泡化。Cur（100mg/kg）给药可使 CPF 暴露动物的血清 LDH 浓度和 OS 生物标志物恢复正常水平（p<0.05），并显著改善心脏损伤。
根据本研究结果，Cur 通过调节 OS 可以减轻 CPF 对大鼠心脏组织的长期暴露的不良影响。
