AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA.
Anticancer Res. 2012 Oct;32(10):4327-30.
Cancer cells, with and without fluorescent protein expression, were irradiated with various doses of UVC (100, 400, and 600 J/m(2)). Dual-color Lewis lung carcinoma cells (LLC) and U87 human glioma cells, expressing GFP in the nucleus and RFP in the cytoplasm and non-colored LLC and U87 cells were cultured in 96-well plates. Eight hours after seeding, the cells were irradiated with the various doses of UVC. The resulting cell number was determined after 24 hours. Compared to non-colored LLC cells, the number of dual-color LLC cells decreased significantly due to UVC irradiation with 100 J/m(2) (p=0.003). Although there was no significant difference in the number of dual-color and non-colored U87 cells after 100 J/m(2) UVC irradiation (p=0.852), the number of dual-color U87 cells decreased significantly with respect to non-colored cells due to UVC irradiation with 400 J/m(2) and 600 J/m(2) (p=0.011 and p=0.009, respectively). Thus, both dual-color LLC and dual-color U87 cells were more sensitive to UVC light than non-colored LLC and U87 cells. These results suggest that the expression of fluorescent proteins in cancer cells can enhance photodynamic therapy (PDT) using UVC and possibly with other wavelengths of light as well.
用不同剂量的 UVC（100、400 和 600 J/m(2)）照射有和没有荧光蛋白表达的癌细胞。培养双荧光素酶标记的 Lewis 肺癌细胞（LLC）和 U87 人神经胶质瘤细胞，使其在核内表达 GFP，在细胞质内表达 RFP，以及非着色的 LLC 和 U87 细胞于 96 孔板中。接种 8 小时后，用不同剂量的 UVC 照射细胞。24 小时后测定细胞数量。与非着色 LLC 细胞相比，双荧光素酶标记的 LLC 细胞由于 100 J/m(2) 的 UVC 照射而数量显著减少（p=0.003）。尽管双荧光素酶标记和非着色 U87 细胞在 100 J/m(2) UVC 照射后数量没有显著差异（p=0.852），但由于 400 J/m(2) 和 600 J/m(2) 的 UVC 照射，双荧光素酶标记的 U87 细胞相对于非着色细胞数量显著减少（p=0.011 和 p=0.009）。因此，双荧光素酶标记的 LLC 和 U87 细胞对 UVC 光比非着色的 LLC 和 U87 细胞更敏感。这些结果表明，荧光蛋白在癌细胞中的表达可以增强使用 UVC 进行的光动力疗法（PDT），并且可能也可以增强其他波长的光的 PDT。
