Chemical Biology Laboratory, National Cancer Institute, Frederick, MD 21702, United States.
Bioorg Med Chem Lett. 2012 Nov 15;22(22):6839-43. doi: 10.1016/j.bmcl.2012.09.055. Epub 2012 Sep 24.
Immunization with whole cells has been used extensively to generate monoclonal antibodies, produce protective immune responses, and discover new disease antigens. While glycans are abundant on cell surfaces, anti-glycan immune responses have not been well-characterized. We used glycan microarrays to profile 49 tumor-binding monoclonal antibodies generated by immunizing mice with whole cancer cells. A substantial proportion (41%) of the tumor binding antibodies bound carbohydrate antigens. The antibodies primarily recognize a group of 5 glycan antigens: Sialyl Lewis A (SLeA), Lewis A (LeA), Lewis X (LeX), blood group A (BG-A), and blood group H on a type 2 chain (BG-H2). The results have important implications for monoclonal antibody production and cancer vaccine development.
用全细胞免疫已被广泛用于产生单克隆抗体、产生保护性免疫反应和发现新的疾病抗原。虽然糖在细胞表面大量存在，但抗糖免疫反应尚未得到很好的描述。我们使用聚糖微阵列对用全癌细胞免疫小鼠产生的 49 种肿瘤结合单克隆抗体进行了分析。相当一部分（41%）的肿瘤结合抗体与碳水化合物抗原结合。这些抗体主要识别一组 5 种糖抗原：唾液酸化路易斯 A（SLeA）、路易斯 A（LeA）、路易斯 X（LeX）、血型 A（BG-A）和 2 型链上的血型 H（BG-H2）。这些结果对单克隆抗体的生产和癌症疫苗的发展具有重要意义。
