糖蛋白疾病标志物和单一蛋白质组学。
Glycoprotein disease markers and single protein-omics.
机构信息
Department of Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, DC 20057, USA.
出版信息
Mol Cell Proteomics. 2013 Apr;12(4):836-45. doi: 10.1074/mcp.R112.026930. Epub 2013 Feb 11.
Abstract
Glycoproteins are well represented among biomarkers for inflammatory and cancer diseases. Secreted and membrane-associated glycoproteins make excellent targets for noninvasive detection. In this review, we discuss clinically applicable markers of cancer diseases and methods for their analysis. High throughput discovery continues to supply marker candidates with unusual glycan structures, altered glycoprotein abundance, or distribution of site-specific glycoforms. Improved analytical methods are needed to unlock the potential of these discoveries in validated clinical assays. A new generation of targeted quantitative assays is expected to advance the use of glycoproteins in early detection of diseases, molecular disease classification, and monitoring of therapeutic interventions.
摘要
糖蛋白是炎症和癌症疾病生物标志物的重要组成部分。分泌型和膜结合型糖蛋白是非常有前途的非侵入性检测靶标。在本综述中，我们讨论了癌症疾病的临床应用标志物及其分析方法。高通量发现不断提供具有异常聚糖结构、糖蛋白丰度改变或特定糖基化位点分布的标记物候选物。需要改进分析方法，以挖掘这些发现应用于经过验证的临床检测的潜力。新一代靶向定量检测有望推进糖蛋白在疾病早期检测、分子疾病分类和治疗干预监测中的应用。
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