Biotechnology and Aquiculture Laboratory, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingangxi Road, Guangzhou, 510300, People's Republic of China.
Mol Biol Rep. 2013 Feb;40(2):1301-6. doi: 10.1007/s11033-012-2173-z. Epub 2012 Oct 14.
It is widely accepted that protein phosphorylation is a major control event in regulating cell cycle. In the present study, a novel M-phase phosphoprotein 6 (MPP6) was identified from black tiger shrimp Penaeus monodon (designated as PmMPP6) by cDNA library and RACE approaches. The full-length cDNA of PmMPP6 was of 690 bp, including a 5'-terminal un-translated region (5'UTR) of 68 bp, a 3'UTR of 172 bp with a poly (A) tail, and an open reading frame (ORF) of 450 bp encoding a polypeptide of 149 amino acids with a predicted molecular weight of 17.01 kDa. Blastx and phylogenetic analysis together supported that PmMPP6 was a novel member of shrimp MPP6. The mRNA expression of PmMPP6 in thirteen tissues was examined by real-time PCR, and mRNA transcript of PmMPP6 was predominantly detectable in tissues of lymphoid and muscle, to a lesser degree in the tissues of gill, ovary and hepatopancreas, and mainly detected in haemocytes, heart and gonad. The temporal expression of PmMPP6 in different developmental stages of ovary was investigated by real-time PCR. During the six stages of ovary development, two peaks expression of PmMPP6 was detected in stage II with 3.78-fold increase and stage V with 3.48-fold increase compared to that in stage I. All these results indicated that PmMPP6 might be involved in regulating shrimp cell cycle and ovary development.
普遍认为，蛋白质磷酸化是调节细胞周期的主要控制事件。本研究采用 cDNA 文库和 RACE 方法，从斑节对虾（Penaeus monodon）中鉴定出一种新型 M 期磷酸蛋白 6（MPP6）（命名为 PmMPP6）。PmMPP6 的全长 cDNA 为 690bp，包括 68bp 的 5′非翻译区（5′UTR）、172bp 的 3′UTR 带有 poly（A）尾，以及 450bp 的开放阅读框（ORF），编码 149 个氨基酸的多肽，预测分子量为 17.01kDa。Blastx 和系统发育分析均支持 PmMPP6 是虾 MPP6 的一个新成员。通过实时 PCR 检测 PmMPP6 在 13 种组织中的 mRNA 表达，结果表明 PmMPP6 mRNA 转录本主要在淋巴和肌肉组织中检测到，在鳃、卵巢和肝胰腺组织中检测到的程度较低，主要在血细胞、心脏和性腺中检测到。通过实时 PCR 研究了 PmMPP6 在卵巢不同发育阶段的时间表达。在卵巢发育的六个阶段中，在第二阶段（增加 3.78 倍）和第五阶段（增加 3.48 倍）检测到 PmMPP6 的两个峰值表达，与第一阶段相比。所有这些结果表明，PmMPP6 可能参与调节虾细胞周期和卵巢发育。
