Mekibeb Abraham, Fulasa Tadele Tolosa, Firdessa Rebuma, Hailu Elena
Department of Veterinary Laboratory Technology, College of Agriculture and Veterinary Science, Ambo University, PO Box 19, Ambo, Ethiopia.
Trop Anim Health Prod. 2013 Mar;45(3):763-9. doi: 10.1007/s11250-012-0287-x. Epub 2012 Oct 12.
A cross-sectional study was conducted on 500 cattle slaughtered at Addis Ababa abattoir to determine the prevalence of bovine tuberculosis (BTB) and characterize its causative agents. Postmortem examination, mycobacteriological culturing, region of difference-4 (RD4)-based PCR and spoligotyping were applied. The prevalence of BTB was 5 % on the basis of postmortem inspection alone but 1.2 % based on molecular confirmation. Factors including age, sex, and breed showed statistically significant association with BTB (p < 0.05). Gross lesions were observed most frequently (68 %) in the lungs and lung-associated lymph nodes compared to other organs and lymph nodes. Of the 25 grossly suspicious TB lesions processed and cultured, only six (24 %) were culture-positive, yielding Mycobacterium bovis confirmed by RD4 deletion typing. Further characterization of the six M. bovis isolates at the strain level by using spoligotyping revealed that one did not belong to any previously known type, while the others belonged to types SB1176 (two), SB1477 (two), and SB0133 (one). The new strain was submitted to the international M. Bovis.org database for international code designation. The study confirms the considerable prevalence of bovine tuberculosis in cattle slaughtered at Addis Ababa abattoir and highlights the need for control of bovine tuberculosis in the country.
对在亚的斯亚贝巴屠宰场宰杀的500头牛进行了一项横断面研究，以确定牛结核病（BTB）的患病率并对其病原体进行特征分析。采用了死后检查、分枝杆菌培养、基于差异区域4（RD4）的聚合酶链反应（PCR）和间隔寡核苷酸分型（spoligotyping）技术。仅基于死后检查，牛结核病的患病率为5%，但基于分子确认则为1.2%。年龄、性别和品种等因素与牛结核病显示出统计学上的显著关联（p < 0.05）。与其他器官和淋巴结相比，肺部及与肺相关的淋巴结中观察到的肉眼可见病变最为常见（68%）。在处理并培养的25个肉眼高度怀疑为结核的病变中，只有6个（24%）培养呈阳性，通过RD4缺失分型确认培养出牛分枝杆菌。通过间隔寡核苷酸分型对6株牛分枝杆菌分离株在菌株水平上进行进一步特征分析，结果显示有1株不属于任何先前已知类型，而其他菌株分别属于SB1176型（2株）、SB1477型（2株）和SB0133型（1株）。新菌株已提交至国际牛分枝杆菌数据库以获取国际代码指定。该研究证实了在亚的斯亚贝巴屠宰场宰杀的牛中牛结核病相当普遍，并强调了该国控制牛结核病的必要性。
