Institute of Hematology, Jinan University, Guangzhou, 510632, China.
Cancer Cell Int. 2012 Oct 15;12(1):42. doi: 10.1186/1475-2867-12-42.
In order to characterize the expression pattern of SALL4, BMI-1 and ABCA3 genes in patients with myeloid leukemia and those who achieved complete remission (CR) after chemotherapy. Real-time PCR was used to determine the expression level of these genes in peripheral blood mononuclear cells from 24 patients with AML, eight patients with AML-CR, 13 patients with CML in the chronic phase (CML-CP), 12 patients with CML in blast crisis (CML-BC), 13 patients with CML-CR and 11 healthy individuals (HI).
Overexpression of the BMI-1 gene was found in the AML, CML-CP and CML-BC groups as compared with HI group, while the BMI-1 expression level was lower in patients who achieved CR. In contrast, significantly increased SALL4 expression was only found in AML group, additionally, SALL4 expression was lower in the CML-CP and CML-CR groups compared with the HI group, while the SALL4 expression level in the CML-BC group was higher and significantly greater than that in the CML-CP and CML-CR groups. Moreover, a positive correlation between the expression of SALL4 and BMI-1 genes was found in samples from most groups. There was no significant difference of ABCA3 expression level in AML and CML-BC group in comparison with HI group. Interestingly, the ABCA3 expression level was significantly decreased in the CML-CP, AML-CR and CML-CR in comparison with the HI group. Moreover, the ABCA3 expression level in all of the CR groups was lower than that in their corresponding groups.
These results describe the altered SALL4, ABCA3 and BMI-1 expression pattern in different phases of myeloid leukemia, which may relate to the development and progression to different diseases. SALL4 expression was strongly correlated with BMI-1 in most of the myeloid leukemia patient groups, providing a potential link between SALL4 and BMI-1 in leukemogenesis.
为了研究 SALL4、BMI-1 和 ABCA3 基因在骨髓性白血病患者及其经化疗达到完全缓解（CR）患者中的表达模式。我们采用实时 PCR 法检测了 24 例 AML 患者、8 例 AML-CR 患者、13 例 CML-CP 患者、12 例 CML-BC 患者、13 例 CML-CR 患者及 11 例健康对照者外周血单个核细胞中这些基因的表达水平。
与健康对照者相比，BMI-1 基因在 AML、CML-CP 和 CML-BC 组中呈过表达，而在获得 CR 的患者中 BMI-1 表达水平较低。相反，仅在 AML 组中发现 SALL4 表达显著增高，此外，与健康对照者相比，SALL4 在 CML-CP 和 CML-CR 组中的表达水平较低，而在 CML-BC 组中的表达水平较高且显著高于 CML-CP 和 CML-CR 组。此外，我们发现大多数组的样本中 SALL4 和 BMI-1 基因的表达呈正相关。与健康对照者相比，AML 和 CML-BC 组的 ABCA3 表达水平无显著差异。有趣的是，与健康对照者相比，CML-CP、AML-CR 和 CML-CR 组的 ABCA3 表达水平显著降低。此外，所有 CR 组的 ABCA3 表达水平均低于其相应组。
这些结果描述了不同阶段骨髓性白血病中 SALL4、ABCA3 和 BMI-1 的表达模式发生改变，这可能与疾病的发生和进展有关。在大多数骨髓性白血病患者组中，SALL4 表达与 BMI-1 强烈相关，这为 SALL4 和 BMI-1 在白血病发生中的潜在联系提供了证据。
