Département de parodontologie, Université Henri Poincaré, Nancy, France.
J Clin Periodontol. 2012 Dec;39(12):1141-8. doi: 10.1111/jcpe.12008. Epub 2012 Oct 16.
This study was designed to evaluate the presence of a new regulator of innate immunity in periodontitis: the soluble form of triggering receptor on myeloid cells-1 (sTREM-1) in gingival crevicular fluid (GCF).
GCF was collected at four sites, three pathological and one healthy from 17 patients with periodontitis, and at one healthy site from 23 control patients. An enzyme-linked immunosorbent assay (ELISA) kit was used to quantify sTREM-1 levels in collected crevicular fluid. Recorded clinical parameters were probing pocket depth (PPD), bleeding upon probing, tooth mobility, plaque index (PlI), and gingival index (GI).
The mean sTREM-1 level in collected fluid was significantly higher in pathological sites than in healthy sites from either periodontal or control patients: 353.9 pg/ml, 50.2 pg/ml and 25.4 pg/ml respectively. Soluble TREM-1 concentration was significantly correlated with PPD. The sTREM-1 levels increased with the augmentation of the PlI and GI scores and levelled off at score 2 for both indexes. In multivariate analysis, periodontal pocket depth and smoking status were statistically associated with highest sTREM-1 concentrations.
sTREM-1 was detected in crevicular fluid and its concentration was higher in pathological sites. It could be a marker of periodontal tissue destruction.
本研究旨在评估一种新的天然免疫调节剂在牙周炎中的存在：龈沟液（GCF）中髓样细胞触发受体-1 的可溶性形式（sTREM-1）。
从 17 名牙周炎患者的四个部位（三个病变部位和一个健康部位）和 23 名对照患者的一个健康部位收集 GCF。使用酶联免疫吸附试验（ELISA）试剂盒定量收集的龈沟液中的 sTREM-1 水平。记录的临床参数包括探诊袋深度（PPD）、探诊出血、牙齿松动、菌斑指数（PlI）和牙龈指数（GI）。
与牙周炎或对照组患者的健康部位相比，病变部位采集的流体中 sTREM-1 的平均水平明显更高：分别为 353.9pg/ml、50.2pg/ml 和 25.4pg/ml。可溶性 TREM-1 浓度与 PPD 显著相关。sTREM-1 水平随着 PlI 和 GI 评分的增加而增加，并且在两个指数的评分 2 时趋于平稳。在多变量分析中，牙周袋深度和吸烟状况与最高 sTREM-1 浓度有统计学关联。
sTREM-1 存在于龈沟液中，其浓度在病变部位较高。它可能是牙周组织破坏的标志物。
