Division of Rheumatology, Department of Internal Medicine, School of Medicine, Marmara University, Istanbul, Turkey.
Department of Health Management, Faculty of Health Sciences, Marmara University, Istanbul, Turkey.
Sci Rep. 2021 Feb 3;11(1):2888. doi: 10.1038/s41598-021-82335-9.
The triggering receptor expressed on myeloid cells 1 (TREM-1) and peptidoglycan recognition protein 1 (PGLYRP1) are involved in the propagation of inflammatory responses. This study investigated whether serum levels of TREM-1 and PGLYRP1 correlate with periodontitis in rheumatoid arthritis (RA) patients. A total of 154 non-smoking participants with RA (n = 55, F/M: 41/14), Behçet´s disease (BD, n = 41, F/M: 30/11) and healthy controls (HC, n = 58, F/M: 40/18) were recruited. Serum and saliva were collected, the 28-joint disease activity score (DAS-28) was calculated and dental/periodontal measurements were recorded. Serum TREM-1 and PGLYRP1 levels were measured by ELISA and salivary bacterial DNA counts by quantitative polymerase chain reaction. TREM-1 and PGLYRP1 levels were higher in RA (166.3 ± 94.3; 155.5 ± 226.9 pg/ml) than BD (102.3 ± 42.8; 52.5 ± 26.3 pg/ml) and HCs (89.8 ± 55.7; 67.4 ± 37.3 pg/ml) (p < 0.05). In RA, periodontitis was associated with increased TREM-1 and PGLYRP1 levels (p < 0.05), yet in patients under methotrexate TREM-1 levels were lower. TREM-1 correlated with C-reactive protein (CRP) levels, DAS-28 and erythrocyte sedimentation rate, whereas PGLYRP1 positively correlated with CRP. RA patients displayed 3.5-fold higher salivary bacterial DNA counts than HCs. Increased serum TREM-1 levels correlated with PGLYRP1, CRP and DAS-28-ESR in RA patients with periodontitis.
髓系细胞触发受体 1（TREM-1）和肽聚糖识别蛋白 1（PGLYRP1）参与炎症反应的传播。本研究探讨了类风湿关节炎（RA）患者的血清 TREM-1 和 PGLYRP1 水平是否与牙周炎有关。共招募了 154 名不吸烟的 RA 患者（n=55，F/M：41/14）、贝切特病（BD，n=41，F/M：30/11）和健康对照者（HC，n=58，F/M：40/18）。收集血清和唾液，计算 28 关节疾病活动度评分（DAS-28），并记录牙齿/牙周测量值。通过 ELISA 检测血清 TREM-1 和 PGLYRP1 水平，通过定量聚合酶链反应检测唾液细菌 DNA 计数。RA 患者的血清 TREM-1（166.3±94.3；155.5±226.9pg/ml）和 PGLYRP1（102.3±42.8；52.5±26.3pg/ml）水平高于 BD（102.3±42.8；52.5±26.3pg/ml）和 HC（89.8±55.7；67.4±37.3pg/ml）（p<0.05）。在 RA 患者中，牙周炎与 TREM-1 和 PGLYRP1 水平升高相关（p<0.05），但接受甲氨蝶呤治疗的患者 TREM-1 水平较低。TREM-1 与 C 反应蛋白（CRP）水平、DAS-28 和红细胞沉降率相关，而 PGLYRP1 与 CRP 呈正相关。RA 患者的唾液细菌 DNA 计数比 HC 高 3.5 倍。在患有牙周炎的 RA 患者中，血清 TREM-1 水平升高与 PGLYRP1、CRP 和 DAS-28-ESR 相关。
