Division of Nutrition and Metabolism, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Clin Nutr. 2013 Jun;32(3):444-51. doi: 10.1016/j.clnu.2012.09.010. Epub 2012 Sep 27.
BACKGROUND & AIMS: Changes in the microbiota composition are able to affect nutrient absorption and energy metabolism, but there are few human studies. The aims were to analyze fecal constituents quantitatively and compare them with liver dysfunction in hepatic cancer patients and to evaluate the relationships among intestinal microbiota, fecal organic acids and plasma lipid composition.
Fecal samples collected from 46 hepatic cancer patients (with liver cirrhosis, chronic hepatitis or liver fibrosis and normal liver) were evaluated for fecal constituents. Blood organic acid, lipid and fatty acid concentrations were analyzed.
Fecal microbiota and organic acids showed no significant differences among different liver dysfunction patients. In normal liver patients, fecal Candida was positively correlated with plasma phospholipid while Bifidobacterium was negatively correlated with plasma eicosapentaenoic acid and eicosapentaenoic acid/arachidonic acid ratio (all p < 0.05). In cirrhotic liver patients, positive correlations were noted for Lactobacillus and docosahexaenoic acid and Candida and eicosapentaenoic acid or eicosapentaenoic acid/arachidonic acid ratio (all p < 0.01). It was suggested that intestinal biota affected serum fatty acid metabolism and were modified by liver disorders.
Intestinal microbiota and organic acid concentrations in hepatic cancer patients had positive and/or negative correlations with serum lipid levels.
微生物组成的变化能够影响营养物质吸收和能量代谢，但目前人类研究较少。本研究旨在分析粪便成分，并将其与肝癌患者肝功能障碍进行比较，同时评估肠道微生物群、粪便有机酸和血浆脂质成分之间的关系。
收集 46 例肝癌患者（伴肝硬化、慢性肝炎或肝纤维化及正常肝脏）的粪便样本，评估粪便成分。分析血有机酸、脂质和脂肪酸浓度。
不同肝功能障碍患者的粪便微生物群和有机酸无显著差异。在正常肝脏患者中，粪便假丝酵母菌与血浆磷脂呈正相关，双歧杆菌与血浆二十碳五烯酸和二十碳五烯酸/花生四烯酸比值呈负相关（均 p<0.05）。在肝硬化患者中，乳杆菌和二十二碳六烯酸与假丝酵母菌和二十碳五烯酸或二十碳五烯酸/花生四烯酸比值呈正相关（均 p<0.01）。提示肠道生物群影响血清脂肪酸代谢，并受肝脏疾病的调节。
肝癌患者肠道微生物群和有机酸浓度与血清脂质水平呈正相关和/或负相关。
