Departments of Surgery and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA.
J Surg Res. 2013 Jun 1;182(1):e1-7. doi: 10.1016/j.jss.2012.09.029. Epub 2012 Oct 6.
The default response of the esophagus to injury includes inflammation and scar tissue formation often leading to stricture. Biologic scaffolds composed of extracellular matrix (ECM) have been associated with the reconstitution of functional esophageal tissue in preclinical studies and clinical case reports of esophageal mucosal resection, anastomotic reinforcement, and full circumferential replacement. However, the mechanisms responsible for this change in the default response to esophageal injury are not fully understood.
The objective of the present study was to determine whether bone marrow-derived cells (BMCs) participate in the long-term remodeling of ECM scaffolds in the esophageal location in a mouse model.
BMCs were present in low numbers in remodeling ECM scaffolds. Compared with the untreated control mice, the ECM-implanted animals showed better remodeling of the epithelial layer.
BMCs are involved in ECM remodeling process during tissue repair after esophageal injury, but the low numbers argue against any significant involvement in the constructive remodeling process.
食管对损伤的默认反应包括炎症和瘢痕组织形成，这通常会导致狭窄。由细胞外基质 (ECM) 组成的生物支架与临床前研究和食管黏膜切除术、吻合口加强和全周替代的临床病例报告中功能性食管组织的重建有关。然而，导致这种对食管损伤的默认反应发生变化的机制尚不完全清楚。
本研究的目的是确定骨髓来源的细胞 (BMCs) 是否参与了在小鼠模型中 ECM 支架在食管部位的长期重塑。
在重塑的 ECM 支架中，BMCs 的数量很少。与未处理的对照组小鼠相比，植入 ECM 的动物表现出更好的上皮层重塑。
BMC 参与了食管损伤后组织修复过程中的 ECM 重塑，但数量很少，表明其在建设性重塑过程中的参与程度有限。
