McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Biomaterials. 2013 Sep;34(28):6729-37. doi: 10.1016/j.biomaterials.2013.05.052. Epub 2013 Jun 15.
Biologic scaffolds composed of extracellular matrix (ECM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical manipulation of the esophagus is often complicated by stricture, but preclinical and clinical studies have shown that the use of an ECM scaffold can mitigate stricture and promote a constructive outcome after resection of full circumference esophageal mucosa. Recognizing the potential benefits of ECM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of ECM from porcine esophageal mucosa. The developed protocol for esophageal ECM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a basement membrane complex. Perivascular stem cells remained viable when seeded upon the esophageal ECM scaffold in-vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.
生物支架由细胞外基质（ECM）组成，常用于促进几种组织（包括食管）的建设性重塑反应。食管的手术操作常常因狭窄而变得复杂，但临床前和临床研究表明，使用 ECM 支架可以减轻狭窄并促进全周食管黏膜切除后的建设性结果。认识到同源组织（即特定部位 ECM）衍生的 ECM 的潜在益处，本研究的目的是制备、表征和评估猪食管黏膜 ECM 的体内重塑特性。开发的食管 ECM 制备方案符合先前建立的脱细胞标准，可得到保留重要生物成分的支架，并具有与基底膜复合物一致的超微结构。血管周干细胞在体外接种到食管 ECM 支架上时仍然存活，而植入软组织时的体内宿主反应显示出建设性重塑的模式。
