透壁电场刺激对大鼠十二指肠肠嗜铬细胞5-羟色胺含量的影响——体外实验

The effect of transmural field stimulation on the serotonin content in rat duodenal enterochromaffin cells--in vitro.

作者信息

Pettersson G, Ahlman H, Dahlström A, Kewenter J, Larsson I, Larsson P A

出版信息

Acta Physiol Scand. 1979 Sep;107(1):83-7. doi: 10.1111/j.1748-1716.1979.tb06445.x.

DOI:10.1111/j.1748-1716.1979.tb06445.x

Abstract

The effect of transmural field stimulation (TFS)--in vitro--on the sertonin (5-HT) content in enterochromafffin cells (EC) in rat duodenum was studied with a cytofluorimetric method. TFS caused a significant 25% decrease of 5-HT in EC. The presence of tetrodotoxin or d,1-propranolol in the stimulation bath antagonized the effect of TFS. In biopsies from rats pretreated with 6-OH-dopamine TFS had no effect of the 5-HT content in EC. The results of the present study strongly suggest, that the TFS induced decrease in 5-HT content is due to a direct neural, probably beta-adrenoceptor mediated, influence on the EC.

摘要

采用细胞荧光测定法研究了透壁电场刺激（TFS，体外）对大鼠十二指肠肠嗜铬细胞（EC）中血清素（5-羟色胺，5-HT）含量的影响。TFS导致EC中5-HT显著降低25%。刺激浴中存在河豚毒素或d，1-普萘洛尔可拮抗TFS的作用。在用6-羟基多巴胺预处理的大鼠活检组织中，TFS对EC中5-HT含量没有影响。本研究结果有力地表明，TFS诱导的5-HT含量降低是由于对EC的直接神经影响，可能是由β-肾上腺素能受体介导的。