Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, Redemption City, Ogun State, Nigeria.
Mol Cell Biochem. 2013 Jan;373(1-2):19-28. doi: 10.1007/s11010-012-1471-z. Epub 2012 Oct 16.
Quercetin has been reported to protect testicular cells from oxidative damage induced by environmental chemicals. In this study, we isolated interstitial Leydig cells (ILCs) from immature rats, set-up ILCs culture, co-treated cells with atrazine (ATZ) and quercetin (QT), evaluated toxicity, and measured the expression levels of antioxidant enzymes and nuclear factor-kappaB (NF-κB) and levels of steroidogenic enzymes. ATZ decreased ILCs viability at concentrations higher than 10 μg/mL and increased reactive oxygen species, malondialdehyde (MDA), and glutathione levels. ATZ also increased glutathione peroxidase, glutathione reductase, and glutathione-S-transferase and decreased superoxide dismutase-1 (sod1) and superoxide dismutase-2 (sod2) messenger RNA (mRNA) levels which were prevented by QT. The changes in the MDA levels and lactate dehydrogenase leakage induced by ATZ (50 μg/mL) were also prevented on co-treatment with QT (50 μM). Furthermore, ATZ-induced 3β- and 17β-hydroxysteroid dehydrogenase activities and NF-κB-expressions at the mRNA and protein levels were also recovered to control value on co-treatment with QT. These data showed that QT protected against ATZ-induced ILCs toxicity by restoring the expression of NF-κB and steroidogenic activity and by preventing the oxidative stress.
槲皮素已被报道可保护睾丸细胞免受环境化学物质诱导的氧化损伤。在这项研究中，我们从未成熟大鼠中分离出间质莱迪希细胞（ILCs），建立 ILCs 培养物，并用莠去津（ATZ）和槲皮素（QT）共同处理细胞，评估毒性，并测量抗氧化酶和核因子-κB（NF-κB）的表达水平以及甾体生成酶的水平。ATZ 在浓度高于 10 μg/mL 时降低 ILCs 的活力，并增加活性氧、丙二醛（MDA）和谷胱甘肽水平。ATZ 还增加了谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶的水平，并降低了超氧化物歧化酶-1（sod1）和超氧化物歧化酶-2（sod2）信使 RNA（mRNA）的水平，QT 可防止这种变化。QT 还可防止 ATZ（50 μg/mL）引起的 MDA 水平和乳酸脱氢酶漏出的变化。此外，QT 还可恢复 ATZ 诱导的 3β-和 17β-羟甾脱氢酶活性以及 NF-κB 在 mRNA 和蛋白质水平的表达，使其恢复至对照值。这些数据表明，QT 通过恢复 NF-κB 和甾体生成活性以及防止氧化应激来保护 ILCs 免受 ATZ 诱导的毒性。
