Suzuki S, Yamasaki K, Katakura Y
Biological Laboratory, Kanagawa Prefectural College, Yokohama, Japan.
Gen Comp Endocrinol. 1990 Feb;77(2):283-91. doi: 10.1016/0016-6480(90)90312-a.
When adult females of Armadillidium vulgare were ovariectomized, the fat body continued to synthesize vitellogenin. On the other hand, females transplanted with androgenic glands decreased the synthetic activity for vitellogenin in their fat body. In order to elucidate effect of the androgenic hormone for vitellogenin synthesis, the occurrence of vitellogenin was studied with andrectomized males. Vitellogenin was estimated, using rocket immunoelectrophoresis. Vitellogenin synthesis in the fat body was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and fluorography. After andrectomy, males ceased elongation of the endopods (copulatory organs) and then vitellogenin was present in their hemolymph. Their vitellogenin titer continued to rise until it reached approximately three times higher than that of normal females. The accumulation level of vitellogenin was comparable with that of ovariectomized females. However, the activity of vitellogenin synthesis was at a low level in the fat body culture of andrectomized males, similar to ovariectomized females. These results indicate that andrectomized males are capable of vitellogenin synthesis, so one of the effects of the androgenic hormone is to inhibit vitellogenin synthesis. In A. vulgare, ovarian factors may not be involved in the induction of vitellogenin synthesis.
当普通鼠妇的成年雌性被切除卵巢后，脂肪体仍继续合成卵黄蛋白原。另一方面，移植了雄性腺的雌性其脂肪体中卵黄蛋白原的合成活性降低。为了阐明雄激素对卵黄蛋白原合成的作用，对去势雄虫的卵黄蛋白原生成情况进行了研究。采用火箭免疫电泳法对卵黄蛋白原进行测定。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和荧光自显影分析脂肪体中卵黄蛋白原的合成。去势后，雄虫的内附肢（交配器官）停止伸长，随后其血淋巴中出现卵黄蛋白原。它们的卵黄蛋白原滴度持续上升，直至达到比正常雌性高约三倍的水平。卵黄蛋白原的积累水平与去卵巢雌性相当。然而，去势雄虫脂肪体培养物中卵黄蛋白原的合成活性处于较低水平，与去卵巢雌性相似。这些结果表明，去势雄虫能够合成卵黄蛋白原，因此雄激素的作用之一是抑制卵黄蛋白原的合成。在普通鼠妇中，卵巢因子可能不参与卵黄蛋白原合成的诱导过程。
