Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju 660-701, Korea.
J Microbiol Biotechnol. 2012 Oct;22(10):1423-31. doi: 10.4014/jmb.1204.04006.
The inhibitory effect of IgY against Vibrio parahaemolyticus and Vibrio vulnificus responsible for seafood-borne diseases was investigated in this study. Water-soluble fractions (WSF) of protein containing IgYs were isolated from the egg yolk of hens initially immunized with formalin inactivated V. parahaemolyticus or V. vulnificus. Protein, total and specific IgY contents of the WSF were determined. The inhibitory and protective effects of IgYs on the growth of V. parahaemolyticus and V. vulnificus were assayed in liquid medium and in mice. IgYs showed high affinity to their corresponding antigens with high titer from day 28 onwards. Protein contents and total IgY concentrations remained stable throughout the immunization period, whereas specific IgY concentrations increased steadily and reached a plateau at day 49. Specific IgY powder (150 mg/ml) significantly inhibited further multiplication of both V. parahaemolyticus and V. vulnificus in liquid medium as compared with the control IgY. The bacteria count in mice feces was lower in mice pretreated with specific IgYs than in those pretreated with PBS or control IgY. Higher survival of mice was observed in the experimental groups pretreated with either anti-V. parahaemolyticus (75% survival) or anti-V. vulnificus (87% survival) IgYs, compared with those in the control groups pretreated with PBS or nonspecific IgY. All mice in the control groups died within three days after bacteria inoculation; hence, the protective effect of specific IgYs against infection caused by V. parahaemolyticus and V. vulnificus was demonstrated.
本研究旨在探讨针对食源性疾病病原菌副溶血性弧菌和创伤弧菌的 IgY 的抑制作用。首先用福尔马林灭活的副溶血性弧菌或创伤弧菌对母鸡进行免疫，从蛋黄中分离出含有 IgY 的水溶性蛋白部分（WSF）。测定 WSF 中的蛋白、总 IgY 和特异性 IgY 含量。在液体培养基和小鼠中检测 IgY 对副溶血性弧菌和创伤弧菌生长的抑制和保护作用。IgY 从第 28 天开始对其相应抗原表现出高亲和力和高滴度。蛋白含量和总 IgY 浓度在整个免疫期间保持稳定，而特异性 IgY 浓度则稳步增加，在第 49 天达到平台期。与对照 IgY 相比，特异性 IgY 干粉（150mg/ml）在液体培养基中能显著抑制副溶血性弧菌和创伤弧菌的进一步增殖。与用 PBS 或对照 IgY 预处理的小鼠相比，用特异性 IgY 预处理的小鼠粪便中的细菌数量更少。用抗副溶血性弧菌（75%存活）或抗创伤弧菌（87%存活）IgY 预处理的实验组小鼠的存活率均高于用 PBS 或非特异性 IgY 预处理的对照组小鼠。所有用 PBS 预处理的对照组小鼠在细菌接种后三天内全部死亡，因此证明了特异性 IgY 对副溶血性弧菌和创伤弧菌感染的保护作用。
