Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
J Neurosci. 2012 Oct 17;32(42):14548-56. doi: 10.1523/JNEUROSCI.0925-12.2012.
We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended-focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amyloid plaques in the brain parenchyma and vasculature and requires no staining of the alzheimeric sample under investigation. xfOCM also opens the possibility to perform minimally invasive studies of β-amyloid pathology in vivo, without the use of labeling methods, which potentially confound experimental findings.
我们使用扩展焦深傅里叶域光学相干显微镜（xfOCM），对阿尔茨海默病的活体小鼠模型和离体脑组织中的脑β-淀粉样蛋白进行无标记成像。xfOCM 提供了大脑实质和脉管系统中单个β-淀粉样斑块的 3D 高分辨率图像，并且不需要对所研究的阿尔茨海默病样本进行染色。xfOCM 还为在不使用潜在干扰实验结果的标记方法的情况下，对活体中的β-淀粉样蛋白病理学进行微创研究开辟了可能性。
