The Institute of Agricultural and Life Science, Chongqing University, Chongqing, 400030, China.
Mol Biol Rep. 2013 Feb;40(2):1407-15. doi: 10.1007/s11033-012-2184-9. Epub 2012 Oct 19.
The mitogen-activated protein (MAP) kinase cascade is an important signaling module which is involved in biotic and abiotic stress responses as well as plant growth and development. In this study, we identified 17 tobacco MAPKs including 11 novel tobacco MAPK genes that have not been identified before. Comparative analysis with MAPK gene families from other plants, such as Athaliana thaliana, rice and poplar, suggested that tobacco MAPKs (such as NtMPK1, NtMPK3 and NtMPK8) might play similar functions in response to abiotic and biotic stresses. QRT-PCR analysis revealed that a total of 14 NtMPKs were regulated by SA and/or MeJA, suggesting their potential roles involved in plant defense response. In addition, 6 NtMPKs were induced by drought treatment, implying their roles in response to drought stress. Our results indicated that most of tobacco MAPK might be involved in plant defense response, which provides the basis for further analysis on physiological functions of tobacco MAPKs.
丝裂原活化蛋白（MAP）激酶级联是一个重要的信号模块，参与生物和非生物胁迫反应以及植物生长发育。在这项研究中，我们鉴定了 17 种烟草 MAPK，包括 11 种以前未鉴定过的新烟草 MAPK 基因。与来自其他植物（如拟南芥、水稻和杨树）的 MAPK 基因家族的比较分析表明，烟草 MAPK（如 NtMPK1、NtMPK3 和 NtMPK8）可能在应对生物和非生物胁迫方面发挥相似的功能。实时定量 PCR 分析显示，共有 14 种 NtMPK 受到 SA 和/或 MeJA 的调控，表明它们在植物防御反应中可能具有潜在的作用。此外，6 种 NtMPK 受到干旱处理的诱导，表明它们在应对干旱胁迫方面的作用。我们的研究结果表明，大多数烟草 MAPK 可能参与植物防御反应，为进一步分析烟草 MAPK 的生理功能提供了基础。
