Zhu Xiaolin, Wang Baoqiang, Wang Xian, Wei Xiaohong
College of Agronomy, Gansu Agricultural University, Lanzhou, China.
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.
Front Genet. 2022 Aug 22;13:961925. doi: 10.3389/fgene.2022.961925. eCollection 2022.
Based on the whole genome data information of , the CqSRS gene family members were systematically identified and analyzed by bioinformatics methods, and the responses of CqSRS genes to NaCl (100 mmol/L), salicylic acid (200 umol/L) and low temperature (4°C) were detected by qRT-PCR. The results showed that a total of 10 SHI related sequence genes were identified in quinoa, and they were distributed on 9 chromosomes, and there were four pairs of duplicated genes. The number of amino acids encoded ranged from 143 aa to 370 aa, and the isoelectric point ranged from 4.81 to 8.90. The secondary structure was mainly composed of random coil (Cc). Most of the SRS gene encoding proteins were located in the cytoplasm (5 CqSRS). Phylogenetic analysis showed that the CqSRS genes were divided into three groups, and the gene structure showed that the number of exons of CqSRS was between two-five. Promoter analysis revealed that there are a total of 44 elements related to plant hormone response elements, light response elements, stress response elements and tissue-specific expression in the upstream regin of the gene. Protein interaction showed that all 10 CqSRS proteins appeared in the known protein interaction network diagram in Arabidopsis. Expression profile analysis showed that CqSRS genes had different expression patterns, and some genes had tissue-specific expression. qRT-PCR showed that all SRS family genes responded to ABA、NaCl、drought and low-temperature treatments, but the expression levels of different CqSRS genes were significantly different under various stresses. This study lays a foundation for further analyzed the function of CqSRS genes.
基于藜麦的全基因组数据信息，采用生物信息学方法对CqSRS基因家族成员进行了系统鉴定和分析，并通过qRT-PCR检测了CqSRS基因对NaCl（100 mmol/L）、水杨酸（200 μmol/L）和低温（4℃）的响应。结果表明，藜麦中共鉴定出10个SHI相关序列基因，它们分布在9条染色体上，存在4对重复基因。编码的氨基酸数量在143个氨基酸至370个氨基酸之间，等电点在4.81至8.90之间。二级结构主要由无规卷曲（Cc）组成。大多数SRS基因编码蛋白位于细胞质中（5个CqSRS）。系统发育分析表明，CqSRS基因分为三组，基因结构显示CqSRS的外显子数量在2至5个之间。启动子分析表明，该基因上游区域共有44个与植物激素响应元件、光响应元件、胁迫响应元件和组织特异性表达相关的元件。蛋白质相互作用表明，所有10个CqSRS蛋白均出现在拟南芥已知的蛋白质相互作用网络图中。表达谱分析表明，CqSRS基因具有不同的表达模式，一些基因具有组织特异性表达。qRT-PCR表明，所有SRS家族基因均对ABA、NaCl、干旱和低温处理有响应，但不同CqSRS基因在各种胁迫下的表达水平存在显著差异。本研究为进一步分析CqSRS基因的功能奠定了基础。
