Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi, 832003, China.
Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou, 571158, China.
Genes Genomics. 2022 Jul;44(7):757-771. doi: 10.1007/s13258-021-01216-7. Epub 2022 Feb 28.
Mitogen-activated protein kinases (MPKs) play important role in response to environmental stress as crucial signal receptors or sensors. Our previous study indicated that salt stress acts as a positive factor to stimulate the production of pharmacodynamic metabolites in the medicinal plant Glycyrrhiza uralensis. Currently, little is known about the MPK gene family and their functions in the medicinal plant G. uralensis.
Identification, comprehensive bioinformatic analysis, expression profiling, and response pattern under salt stress of the G. uralensis GuMPK gene family.
Genome-wide investigation and expression profiling of the MPK gene family in G. uralensis, and their phylogenetic relationships, evolutionary characteristics, gene structure, motif distribution, promoter cis-acting element, and expression pattern under salt stress in two different salt-tolerant Glycyrrhiza species were performed.
A total of 20 G. uralensis GuMPK genes were identified and categorized into five groups, and had conserved gene structure and motif distribution. Expression profiling of GuMPK genes suggested their potentially diverse functions in plant growth and in response to phytohormones and environmental stress, particularly GuMPK1, 2, 5, and 10 as key components for G. uralensis in response to abiotic stress. Further expression analysis under NaCl treatment in two different salt-tolerant Glycyrrhiza species displayed the MPKs' different response patterns, emphasizing the role of MPK2, 5, 7, and 16 as potentially crucial genes for Glycyrrhiza to respond to salt stress.
Our results provide a genome-wide identification and expression profiling of MPK gene family in G. uralensis, and establish the foundation for screening key responsive genes and understanding the potential function and regulatory mechanism of GuMPKs in salt responsiveness.
丝裂原活化蛋白激酶（MPKs）作为重要的信号受体或传感器，在应对环境胁迫方面发挥着重要作用。我们之前的研究表明，盐胁迫作为一种积极因素，刺激药用植物甘草中药效代谢物的产生。目前，关于药用植物甘草中的 MPK 基因家族及其功能知之甚少。
鉴定、综合生物信息学分析、表达谱分析以及盐胁迫下药用植物甘草 GuMPK 基因家族的响应模式。
对甘草基因组进行了广泛的 MPK 基因家族调查和表达谱分析，分析了它们的系统发育关系、进化特征、基因结构、基序分布、启动子顺式作用元件以及在两种不同耐盐甘草物种中的盐胁迫下的表达模式。
共鉴定出 20 个甘草 GuMPK 基因，分为 5 组，具有保守的基因结构和基序分布。GuMPK 基因的表达谱分析表明，它们在植物生长和对植物激素及环境胁迫的响应中具有潜在的多样化功能，特别是 GuMPK1、2、5 和 10 作为甘草响应非生物胁迫的关键组成部分。进一步对两种不同耐盐甘草物种在 NaCl 处理下的表达分析显示，MPKs 表现出不同的响应模式，强调了 MPK2、5、7 和 16 作为甘草响应盐胁迫的潜在关键基因的作用。
本研究提供了甘草中 MPK 基因家族的全基因组鉴定和表达谱分析，为筛选关键响应基因和理解 GuMPKs 在盐响应中的潜在功能和调控机制奠定了基础。
