Brigham Christopher J, Zhila Natalia, Shishatskaya Ekaterina, Volova Tatiana G, Sinskey Anthony J
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
Subcell Biochem. 2012;64:343-66. doi: 10.1007/978-94-007-5055-5_17.
Ralstonia eutrophais a Gram-negative betaproteobacterium found natively in soils that can utilize a wide array of carbon sources for growth, and can store carbon intracellularly in the form of polyhydroxyalkanoate. Many aspects of R. eutrophamake it a good candidate for use in biotechnological production of polyhydroxyalkanoate and other bio-based, value added compounds. Manipulation of the organism's carbon flux is a cornerstone to success in developing it as a biotechnologically relevant organism. Here, we examine the methods of controlling and adapting the flow of carbon in R. eutrophametabolism and the wide range of compounds that can be synthesized as a result. The presence of many different carbon utilization pathways and the custom genetic toolkit for manipulation of those pathways gives R. eutrophaa versatility that allows it to be a biotechnologically important organism.
嗜麦芽窄食单胞菌是一种革兰氏阴性β-变形菌，天然存在于土壤中，它能够利用多种碳源进行生长，并能以聚羟基脂肪酸酯的形式在细胞内储存碳。嗜麦芽窄食单胞菌的许多特性使其成为用于聚羟基脂肪酸酯及其他生物基增值化合物生物技术生产的良好候选对象。操纵该生物体的碳通量是将其开发成为具有生物技术相关性生物体取得成功的基石。在此，我们研究了控制和调节嗜麦芽窄食单胞菌代谢中碳流的方法以及由此可合成的多种化合物。许多不同碳利用途径的存在以及用于操纵这些途径的定制遗传工具包赋予了嗜麦芽窄食单胞菌多功能性，使其成为具有生物技术重要性的生物体。
