Technische Universität Berlin, Institute of Biotechnology, Chair of Bioprocess Engineering, Berlin, Germany.
Berliner Hochschule Für Technik, Department VIII - Mechanical Engineering, Event Technology and Process Engineering, Environmental and Bioprocess Engineering Laboratory, Berlin, Germany.
Appl Microbiol Biotechnol. 2024 Aug 29;108(1):450. doi: 10.1007/s00253-024-13284-2.
Synthetic biology encompasses many kinds of ideas and techniques with the common theme of creating something novel. The industrially relevant microorganism, Ralstonia eutropha (also known as Cupriavidus necator), has long been a subject of metabolic engineering efforts to either enhance a product it naturally makes (polyhydroxyalkanoate) or produce novel bioproducts (e.g., biofuels and other small molecule compounds). Given the metabolic versatility of R. eutropha and the existence of multiple molecular genetic tools and techniques for the organism, development of a synthetic biology toolkit is underway. This toolkit will allow for novel, user-friendly design that can impart new capabilities to R. eutropha strains to be used for novel application. This article reviews the different synthetic biology techniques currently available for modifying and enhancing bioproduction in R. eutropha. KEY POINTS: • R. eutropha (C. necator) is a versatile organism that has been examined for many applications. • Synthetic biology is being used to design more powerful strains for bioproduction. • A diverse synthetic biology toolkit is being developed to enhance R. eutropha's capabilities.
合成生物学包含许多具有创造新颖事物这一共同主题的想法和技术。具有工业相关性的微生物罗尔斯通氏菌（也称为醋杆菌属）一直是代谢工程努力的主题，这些努力旨在增强其天然产生的产物（聚羟基脂肪酸酯）或生产新型生物制品（例如，生物燃料和其他小分子化合物）。鉴于罗尔斯通氏菌的代谢多功能性，以及该生物体存在多种分子遗传工具和技术，因此正在开发一种合成生物学工具包。该工具包将允许进行新颖的、用户友好的设计，为罗尔斯通氏菌菌株赋予新的能力，用于新型应用。本文综述了目前可用于修饰和增强罗尔斯通氏菌生物生产的不同合成生物学技术。要点：•罗尔斯通氏菌（C. necator）是一种多功能的生物体，已经被研究用于许多应用。•合成生物学正被用于设计更强大的生物生产菌株。•正在开发多样化的合成生物学工具包以增强罗尔斯通氏菌的能力。
